人纤溶酶原Kringle1基因的克隆与表达  

Cloning and Expression of Human Plasminogen Kringle 1

作　　者：陶亦敏[1] 唐亮 张颉 谈立松 陈宇光[1] 

机构地区：[1]上海大学生命科学学院,上海200436 [2]上海市第一肺科医院,上海200433

出　　处：《上海大学学报（自然科学版）》2002年第6期548-551,556,共5页Journal of Shanghai University:Natural Science Edition

摘　　要：从人肝组织中抽提总RNA,通过RT-PCR方法扩增出人纤溶酶原(plasminogen)Kringle1片段,构建pPIC9K-K1载体,电击转化酵母,成功获得pPIC9K-K1/GS115工程菌.摇瓶发酵初步结果表明,工程菌能稳定表达可溶性的重组K1蛋白,分子量为15kD,发酵液中目的蛋白含量为84.17mg/L.Total RNA was extracted from human fresh liver. The gene encoding Kringle 1 of plasminogen was amplified by RTPCR. Then Kringle 1 gene was introduced into vector pPIC9K. Yeast cell GS115 was transfected by linear recombinant plasmid. Finally the yeast engineering cells named pPIC9KK1/GS115 was obtained stably expressing Kringle 1. Flask test showed that recombination protein K1 in culture supernatants reached up to 84.17 mg/L.

关 键 词：人纤溶酶原 KRINGLE 1基因 克隆 表达 抗肿瘤药物 

分 类 号：R979.1[医药卫生—药品] Q78[医药卫生—药学]