猪肝细胞的Gerlach分离法  

The Gerlach Isolation of Porcine Hepatocytes

作　　者：刘应生[1] 胡以则[2] 林景泰[2] 章乐虹[2] 杨学伟[2] 向荣超[2] 

机构地区：[1]广州医学院荔湾医院普外科,广州510170 [2]广州医学院第二附属医院肝胆外科,广州510260

出　　处：《广州医学院学报》2002年第3期54-57,共4页Academic Journal of Guangzhou Medical College

摘　　要：目的 :探讨猪肝细胞分离收获率、存活率。方法 :取本地杂种小猪作为肝细胞供体 ,采用Gerlach五步胶原酶灌流法分离猪肝细胞 ,血细胞计数板计数细胞产量 ,TrypanBlue拒染法计算细胞活率。结果 :每只猪肝平均可获得 (12 .2 2± 1.5 7)× 10 9个肝细胞 ,平均活力为 95 .4 %± 2 .4 1%。结论 :采用Gerlach五步胶原酶灌流法分离猪肝细胞 。Objective:To study the yield and viability of porcine hepatocytes isolation. Methods:Porcine hepatocytes were isolated by the modified five step collagenase perfusing method developed by Gerlach. The yield and viability were assessed by Trypan Blue exclusion. Results:The average yield of porcine hepatocytes was (12.22±1.57)×10 9 cells per pig with an average viability of 95.4±2.41%. Conclusion:The isolation of porcine hepatocytes by five step enzymetic method can be harvested with high yield and viability.

关 键 词：猪肝细胞 细胞分离 生物人工肝 Gerlach分离法 

分 类 号：R657.3[医药卫生—外科学]