TUNEL法检测新风胶囊对AA大鼠滑膜胸腺及胃粘膜细胞凋亡的影响  

The Influence on Xinfeng capsule(新风胶囊)on the apoptosis of the Synoviocyte and thymus-lymphocyte of Adjurant Arthritis Rat by ter minal deoxynuleotidyl transferase mediated Dutp nick end labeling(TUNEL)

作　　者：刘健[1] 张梅奎[2] 韩明向[1] 翟志敏[3] 刘晓晖[1] 

机构地区：[1]安徽中医学院第一附属医院,安徽合肥230031 [2]解放军第304医院,北京100029 [3]安徽省立医院,安徽合肥230001

出　　处：《中医药学刊》2003年第1期54-56,共3页Study Journal of Traditional Chinese Medicine

摘　　要：目的 :观察以终末脱氧核糖核酸转移酶介导的原位缺口末端标记 (ter分钟aldeoxynucleotidyltrans ferasemediatedDUTPnickendlabeling ,TUNEL)技术检测新风胶囊对佐剂性关节炎 (adjuvantarthritis ,AA)大鼠滑膜、胸腺及胃粘膜细胞凋亡的影响 ,探讨新风胶囊的作用机制。方法 :采用弗氏完全佐剂造成佐剂性关节炎 (AA)大鼠模型 ,设立正常对照组、模型对照组、甲氨喋呤 (MTX)对照组、雷公藤多甙 (TriperygiumWilfordiiPolycorideTablet,TPT)对照组和新风胶囊 (XinfengCepsule ,XFC)治疗组。记录各组大鼠的关节炎指数及体重变化 ,应用流式细胞仪 (FCM)以TUNEL法测定各组大鼠滑膜、胸腺及胃粘膜细胞凋亡率。结果 :与治疗前相比 ,XFC、TPT及MTX组治疗后大鼠的关节炎指数显著降低 (P <0 .0 5～ 0 .0 1) ,XFC组治疗后AA大鼠体重显著增加 (P <0 .0 5 ) ,而TPT及MTX组体重无显著变化 (P >0 .0 5 ) ;模型组滑膜及胸腺细胞凋亡率显著低于、胃粘膜细胞凋亡率显著高于正常对照组 (P <0 .0 5 ) ,XFC组滑膜及胸腺细胞凋亡率显著高于、胃粘膜细胞凋亡率显著低于模型组、TPT及MTX组 (P <0 .0 5～ 0 .0 1)。结论 :XFC与MTX、TPT一样能降低AA大鼠关节炎指数 ,在增加AA大鼠体重、促进滑膜、胸腺细胞凋亡及抑制胃粘膜细胞凋亡方?Objective:To observe the effect of Xinfeng capsule(XFC,新风胶囊)on the apoptosis of the synoviocyte?thymus-lymphocyte and gastric-mucosa cell of adjuvant arthritis (AA)rat by TUNEL.Methods:Model rats were established by the Freund's complete adjuvant.Fifty rats were randomly divided into 5 groups:normal control?model control?methotrexate(MTX)control?triperygium wilfordii polycoride tablet (TPT)control?xinfeng capsule treated(XFC).The arthritis index(AI)and weight of rats was recorded.The apoptotic percentage of the synoviocyte?thymus-lymphocyte and gastric-mucosa cell of AA was measured by flow cytometry (FCM)using TUNEL.Results:Compared with before treatment,XFC?MTXand TPT can decrease the AI(P<0.01～0.05),the weight of rats in XFC increased markedly after treatment(P<0.05)and the weight of rats in TPT and MTX had no marked change(P>0.05).Compared with the normal control,apoptotic percentage of the synoviocyte and thymus-lymphocyte of model control was decreased and apoptotic percentage of gastric-mucosa cell of model control was increased(P<0.05);Compared with the MTX?TPT and model control,apoptotic percentage of the synoviocyte and thymus-lymphocyte of XFC was significant increased and apoptotic percentage of gastric-mucosa cell of XFC was decreased(P<0.01～0.05).Conclusion:XFC can decrease the AI like MTX?TPT.But it is superior to MTX?TPT in increasing the weight of rats and the apoptosis of the synoviocyte and thymus-lymphocyte,decreasing the apoptosis of the gastric-mucosa cell of AA.It maybe the mechanism for XFC that promot the apoptosis of synoviocyte and thymus-lymphocyte,restrain the synovial membrane from hyperplasia and autoimmune reaction,restrain the apoptosis of gastric-mucosa cel and protect gastric mucosa. 〔

关 键 词：新风胶囊 佐剂性关节炎 细胞凋亡 中药 药理 

分 类 号：R285.5[医药卫生—中药学]