用RAPD技术分析中国嗜人按蚊种型  

SPECIES ANALYSIS ON ANOPHELES ANTHROPOPHAGUS IN CHINA WITH RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION METHOD

作　　者：金玉明[1] 王善青[1] 王赛梅[1] 李善干[1] 杨霞[1] 

机构地区：[1]海南省热带病防治研究所,海南海口570203

出　　处：《中国寄生虫病防治杂志》2002年第6期328-330,共3页Chinese Journal of Parasitic Disease Control

基　　金：海南省自然科学基金资助项目 (No.3981 6)~~

摘　　要：目的　鉴别中国不同地区嗜人按蚊 ,特别是海南岛与内陆嗜人按蚊间有无种型差异。　方法　海南、四川、江苏三地收集嗜人按蚊标本 ,通过对蚊基因组 DNA的提取纯化及 RAPD- PCR扩增和产物检测 ,最终筛选出 3条引物并制定出稳定的 RAPD图谱。　结果　三地嗜人按蚊绝大部分谱带完全相同 ,但又有明显不同的谱带存在。　结论　中国不同地区嗜人按蚊存在一定的种型差异 ,并为解释海南岛与内陆嗜人按蚊在生态习性和传疟作用方面的不同提供了科学依据。Objective To identify if there are the species' difference among the strains of Anopheles anthropophagus of different areas, especially between Hainan Island and the inner land in China. Methods The samples of An. anthropophagus were collected from Hainan Island, Sichuan Province and Jiangsu Province, and cultured in the laboratory. Through extraction and purification of mosquito genomic DNA, amplification with RAPD PCR technique and detection and recording of amplified fragments, finally 3 primers were screened out and the stable RAPD maps were obtained. Results Most of the amplified fragments were common for the three areas' An. anthropophagus , but some fragments were unique to one area's An. anthropophagus . Conclusion There were the species difference of An. anthropophagus in China, that might explain why the ecological habits and malaria transmitting roles of An. anthropophagus in Hainan Island were different from that in the inner land.

关 键 词：随机扩增多态DNA聚合酶链反应 嗜人按蚊 引物 种型 

分 类 号：R384.1[医药卫生—医学寄生虫学]