一种裂体吸虫与日本血吸虫蛋白质差异研究  

STUDY ON DIFFERENCES OF PROTEIN BETWEEN SCHISTOSOMA X AND SCHISTOSOMA JAPONICUM

作　　者：徐国余[1] 陈广梅[1] 田海生[2] 朱昌亮[2] 

机构地区：[1]江苏省南京市疾病预防控制中心,南京210003 [2]南京医科大学病原生物学与免疫学系

出　　处：《中国血吸虫病防治杂志》2002年第6期405-406,共2页Chinese Journal of Schistosomiasis Control

摘　　要：目的　探讨裂体吸虫 x(S.x)和日本血吸虫 (S.j)的蛋白质有无差异。方法　采用 SDS-PAGE法。感染了 S.x和 S.j尾蚴的兔 45 d后剖杀 ,从肠系膜静脉血管中取出成虫 ,各 2 0对 ,匀浆 ,高速离心后取上清液 ,电泳 ,分离胶和浓缩胶分别为 10 %和 4%。电泳完成后以考马斯兰 R2 50 染色。应用分子分析软件系统 Gel Doc10 0 0 (BIO-RAD)进行分析 ,并计算出 S.x与 S.j的比值 (S.x/ S.j)。结果　 S.x和 S.j蛋白带各有 2 6条 ,其中 3 5 .0 k Da和 10 9.8k Da为 S.x和 S.j分别特有的蛋白带 ,它们之间存在明显的差异 ;S.x和 S.j的 87.3 k Da,40 .9k Da和 3 2 .2 k Da蛋白质量差异达 2倍。 15 .2 k Da蛋白质量差异 1.9倍 ,73 .2 k Da蛋白质量差异 1.7倍。结论　 S.x和 S.j成虫可溶性蛋白所分出的带数经定性、定量分析 。Objective To study the differences in expressive protein between Schistosoma x (S.x) and Schistosoma japonicum (S.j). Methods Twenty pairs of worms were collected from rabbits infected with cercariae of S.x and S.j respectively. SDS PAGE was performed on Hoefer 250 (Hoefer, USA) according to the manufactures instruction. The samples were run on 10% sodium dodecylsulfate polyacrylamide gels (SDS PAGE) under the condition of 20 mA (constant currant) for 1 h. After electrophoresis, gels were stained by Coomassie Brilliant Blue R250. The electrophoresis pattern of protein of S.x and S.j. by SDS PAGE were analysis by software of Labworks TM . Results There were different bands of expressive proteins between S.x and S.j. Among them, the 35 0 kDa band was specific in S.x and 109 8 kDa band was expressed only in S.j. There were 3 bands, 87 3 kDa, 40 9 kDa and 32 2 kDa, of expressive protein which had significant difference (2 times) in quantity between S.x and S.j, and 2 bands, 15 2 kDa and 73 2 kDa, difference (1 9 and 1 7 times) between S.x and S.j. Conclusion The differences of protein fractions between S.x and S.j may be associated with different species.

关 键 词：血吸虫x 日本血吸虫 蛋白质 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 

分 类 号：R383.24[医药卫生—医学寄生虫学]