大鼠伏隔核神经元膜特性及乙醇对其影响  

Membrane properties of rat nucleus accumbens septi neurons and the effects of ethanol on them

作　　者：朱学江[1] 张小虎[1] 朱维[1] 戈应滨[1] 袁孝如[1] 

机构地区：[1]南京医科大学生理学教研室,江苏南京210029

出　　处：《中国神经科学杂志》2003年第1期1-4,共4页

基　　金：江苏省教委基金 (0 0KJB3 10 0 0 5 )

摘　　要：目的 :观察伏隔核神经元的膜特性及致醉剂量的乙醇 (44mmol L)对其影响。 方法 :在大鼠伏隔核脑片上 ,采用细胞内电流钳记录技术。结果 :所观察到的神经元包括五种类型 ,神经元的静息膜电位 (mV)为 - 79.1± 2 .4 ,输入阻抗 (MΩ)为 4 8.4± 2 .3,锋电位的幅度 (mV)平均为 98.5± 4 .1;致醉剂量的乙醇 (44mmol L)对大部分伏隔核神经元的膜电位、输入阻抗及I V曲线无明显影响。 结论 :在伏隔核内 ,存在多种膜特性类型的神经元 ;致醉剂量的乙醇对大鼠伏隔核神经元的膜特性无明显影响 ,说明乙醇对伏隔核神经元的抑制作用主要并不是通过影响膜特性来实现的。Objective:To observe SD rat nucleus accumbens septi (NAcc) neuronal membrance properties and the effects of ethanol on them. Methods: Intracellular recording and current clamp technique were used.Results: The observed neurons had five different kinds,many of them had large resting membrance potential( averaging -79.1 mV±2.4 mV),their input resistance was 48.4 MΩ±2.3 MΩ and evoked spikes was 98.5 mV±4.1 mV. After superfusion of intoxicating doses of ethanol (44 mmol/L),the average resting membrance potential was 80.3 mV±2.1 mV( vs control P >0.05 ) and input resistance was 48.7 MΩ±2.1 MΩ ( vs control P >0.05), only 2 neurons showed a hyperpolaring response. Conclusion: Superfusion of intoxicating dose of ethanol had little effect on membrane potential or input resistance.

关 键 词：伏隔核 脑片 电流钳技术 乙醇 

分 类 号：R338[医药卫生—人体生理学]