皮下注射多聚甲醛诱导大鼠脊髓和三叉神经脊束核向延髓网状背侧亚核传入投射的fos表达  

Subcutaneous injection of paraformaldehyde induces Fos expression in neurons of spinal cord and the trigeminal subnucleus caudalis projecting to the subnucleus reticularis dorsalis in the rat

作　　者：高永静[1] 凌树才[2] 倪衡建[1] 朱鸣镝[3] 

机构地区：[1]南通医学院神经生物学研究室 [2]浙江大学医学院人体解剖学教研室,浙江杭州310031 [3]南通医学院附属医院骨科,江苏南通226001

出　　处：《中国神经科学杂志》2003年第1期13-16,共4页

基　　金：江苏省自然科学基金资助项目 (BK9715 0 )

摘　　要：目的 :研究外周伤害性刺激下大鼠脊髓和三叉神经脊束核尾侧亚核向延髓网状背侧亚核传入投射的神经元中的Fos表达。方法 :采用荧光金逆行追踪及Fos免疫组化染色相结合的方法。结果 :脊髓和三叉神经脊束核尾侧亚核均有神经元投射到延髓网状背侧亚核。在颈髓背角浅层 ,FG Fos双标神经元分别占FG阳性神经元和Fos阳性神经元的 9.3%和 7.5 % ;在三叉神经脊束核尾侧亚核浅层 ,FG Fos双标神经元分别占FG阳性神经元和Fos阳性神经元的 9.5 %和 14 .2 %。结论 :在颈髓背角浅层和三叉神经脊束核尾侧亚核浅层向延髓网状背侧亚核传入投射的神经元中 ,部分神经元可能与伤害性刺激有关。Objective: To investigate Fos expression in the neurons of spinal cord and the trigeminal subnucleus caudalis (Vc) projecting to the subnucleus reticularis dorsalis (SRD) under peripheral noxious stimulation in the rat. Methods: Fluorogold (FG) retrograde combined with fluorescent immunohistochemistry was used. Results: There were neurons projecting to SRD from spinal cord and Vc. In spinal cord, the percentages of FG/Fos double labeled neurons in the population of FG positive and Fos positive neurons were 9.3% and 7.5%, respectively; In trigeminal subnucleus caudalis, they were 9.5% and 14.2%, respectively. Conclusion: Some afferent neurons to SRD in spinal cord and Vc may be involved in transmission of peripheral noxious signals. \[

关 键 词：延髓网状背侧亚核 脊髓 三叉神经脊束核尾侧亚核 荧光金 FOS 大鼠 

分 类 号：R338[医药卫生—人体生理学]