氧疗对慢性低氧高二氧化碳性肺动脉高压大鼠肺血管重构及血浆内皮素的影响  

Influence of oxygen therapy on pulmonary vessel remodeling and plasma ET-1 level in rats with pulmonary hypertension induced by chronic hypoxia-hypercapnia

作　　者：范小芳[1] 龚永生[1] 胡良冈[1] 刘道侯[1] 李继武[1] 陈静炯[1] 

机构地区：[1]温州医学院肺心病研究室,浙江温州325027

出　　处：《温州医学院学报》2002年第6期348-350,共3页Journal of Wenzhou Medical College

基　　金：浙江省卫生厅科研基金资助项目

摘　　要：目的 :探讨长期氧疗对慢性低氧高二氧化碳性肺动脉高压大鼠肺动脉压、肺血管重构及血浆内皮素变化的影响。方法 :将SD大鼠分 4组 :正常对照组 (NC)、慢性低氧 4w后伴高二氧化碳 2w组 (HH)、HH后吸空气 3w组 (HC)与HH后氧疗 3w组 (HO)。观察各组大鼠平均肺动脉压 (mPAP)、右心室 /左心室 +室间隔重量比 (RV/LV +S)、血浆内皮素 1(ET 1)浓度及肺细小动脉显微结构变化。结果 :①mPAP、RV/LV +S及血浆ET 1浓度 :HO组比HC组明显降低 (P <0 .0 1)。②mPAP与血浆ET 1呈良好的相关性。③光镜下HH组肺细小动脉内弹力板扭曲 ,中膜平滑肌细胞增生 ,管腔明显狭窄 ;HO组肺细小动脉内弹力板扭曲明显减轻 ,中膜平滑肌层变薄 ,管壁较均匀一致。结论 :长期氧疗可明显降低慢性低氧高二氧化碳肺动脉高压大鼠血浆ET 1的浓度及肺动脉压 ,减轻肺血管的重构。Objective:To investigate the role of oxygen therapy on pulmonary vascular remodeling and plasma ET 1 level in rats with chronic hypoxia and hypercapnia induced pulmonary hypertension.Methods:Changes in mean pulmonary artery pressure(mPAP),weight ratio of right ventricle to left ventride and septum(RV/LV+S),pulmonary arterial microscopy and plasma ET 1 were observed. Forty male Sprague Dawley rats were randomly divided into four groups:normal control group(NC),groups of only 2 week hypoxia hypercapnia(HH),2 week hypoxia hypercapnia and 3 week oxygen therapy(HO) and 2 week hypoxia hypercapnia and 3 week air inhalation(HC),hypoxia after 4 week.Results:①mPAP and RV/LV+S of HH group were higher than those of NC group(P<0.01). In HO group,mPAP and RV/LV+S were lower(P<0.05) than those in HC group.②Level of plasma ET 1 in HH group was significantly higher than that in NC group(P<0.01),but the level of plasma ET 1 of HO group was lower than that of HC group.③Light microscopy showed that vessel well area/total area(WA/TA),and media thickness of pulmonary arterioles were much higher in rats of HH group and vessel cavity area/total area(EA/TA) was lower in HH group than those in NC group(P<0.01). WA/TA,EA/TA and mMTPA in HO group were obviously improved.Conclusion:Oxygen therapy may have great effect on pulmonary artery pressure and plasma ET 1 level. It can obviously decrease the pulmonary arterial remodeling.

关 键 词：内皮素 肺性高血压 低氧 

分 类 号：R543.2[医药卫生—心血管疾病] R594.[医药卫生—内科学]