丹仙康骨胶囊对培养成骨细胞增殖分化的影响  

Effects of Danxian Kanggu Capsule on Proliferation and Differentiation of Cultured Osteoblasts in Vitro

作　　者：刘日光[1] 沈冯君[1] 易诚青[1] 刘建湘[1] 

机构地区：[1]华中科技大学同济医学院协和医院骨科,湖北武汉430022

出　　处：《辽宁中医杂志》2003年第1期76-77,共2页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的 :为了解补肾活血中药丹仙康骨胶囊对体外培养成骨细胞的作用。方法 :应用MTT ,对硝基苯磷酸盐法及骨钙素含量放免测定法 ,观察丹仙康骨胶囊对成骨细胞增殖与分化作用的影响。结果 :丹仙康骨胶囊刺激成骨细胞增殖 ,提高ALP活性及骨钙素含量。结论Objective :To investigate the effects of Danxian Kanggu(DXKG)capsule extracted from traditional Chinese medicine on cultured osteoblasts in vitro. Methods :MTT,PNPP and BGP content radioimmunoassay were used to observe the proliferation,the activity of ALP and the BGP content of osteoblasts cultured in vitro. Results :It was found that DXKG capsule had the effects on stimulating the proliferation,ALP activity and the BGP content of cultured osteoblasts in different concentration. Conclusion :DXKG capsule has effects on stimulating the proliferation and the differentiation,and increasing the activity of ALP and the BGP content of cultured osteoblasts in vitro.

关 键 词：丹仙康骨胶囊 细胞培养 成骨细胞 中药药理 

分 类 号：R285.5[医药卫生—中药学]