铜绿假单胞菌临床分离株gyrA基因突变与喹诺酮类耐药关系研究  

Studies on relationship between gyrA gene mutation and quinolone resistance of Pseudomonas aeruginosa

作　　者：宋世平[1] 黄春基[1] 张中奎[1] 解小珍 

机构地区：[1]第三军医大学第二附属医院检验科,重庆400037

出　　处：《临床检验杂志》2003年第1期12-14,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的　了解铜绿假单胞菌临床分离株 gyrA基因突变与喹诺酮类药物耐药的关系 ,研究其临床意义。 方法　通过PCRHinfⅠ酶切分析、PCR 单链构象多态性分析 (SSCP)等方法检测铜绿假单胞菌ATCC 2 785 3及 36株铜绿假单胞菌临床分离株的 gyrA基因突变情况。 结果　HinfⅠ酶切结果显示 ,所有 13株环丙沙星耐药株 ,3株环丙沙星中介株中的 2株 ,2 0株环丙沙星敏感株中的 1株存在 gyrA基因HinfⅠ酶切位点突变 ;SSCP分析结果显示 ,所有耐药菌株和中介菌株的 gyrA基因带型与标准菌株均不同 ;2 0株敏感菌株中有 5株的带型与标准菌株不同。结论　铜绿假单胞菌喹诺酮类耐药与 gyrA基因HinfⅠ酶切位点突变密切相关 ,但 gyrA基因突变不局限于该位点 ,在常规药敏试验的基础上开展Objective To observe the relationship between gyrA gene mutations and quinolone resistance of Pseudomonas aeruginosa and explore its clinical significance.Methods Mutations of gyrA gene were analysed with PCR RFLP and PCR SSCP in 36 clinical Pseudomonas aeruginosa strains and strain ATCC27853.Results The results of PCR RFLP showed that all 13 quinolone senstive strains,2 quinolone intermediate resistant strains and 1 quinolone sensitive strains exhibited gyrA gene mutations in Hinf I digestion.PCR SSCP showed that the genotype of all quinolone resistant strains and strain ATCC27853 were different from the standard strain.Conclusions There is a close relationship between quinolone resistance and the mutation of restriction site of gyr A gene by Hinf I digestion in Pseudomonas aeruginosa ,but the mutations do not be just limited in gyrA gene Hinf I digestion site.The findings in this study has important significance in detection of the gyrA gene mutations on the basis of routine drug susceptibility test of antibiotics.

关 键 词：铜绿假单胞菌 GYRA基因 突变 喹诺酮 

分 类 号：R378.991[医药卫生—病原生物学]