银杏内酯A、B对豚鼠乳头肌细胞的电生理效应  

The Electrophysiological Effect of Ginkgolide A、B on the Papillary Muscle of Guinea pig

作　　者：张志雄[1] 祁小燕[1] 王星禹[1] 崔启启[2] 施渭彬[2] 

机构地区：[1]上海中医药大学生理教研室,220321 [2]上海第二医科大学

出　　处：《中西医结合心脑血管病杂志》2003年第1期2-4,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：上海市教委科学技术发展基金项目 (NO .99G1 8)

摘　　要：目的 :研究银杏内酯A、B(GA、GB)抗心肌缺血与抗心律失常的效应及其机制。方法 :细胞内微电极方法记录乳头肌动作电位。灌流模拟缺血液造缺血模型以及在低钾台氏液中加入氯化铯灌流诱发延迟后除极 (DAD)模型。观察GA、GB在生理、缺血状态下对乳头肌动作电位各项电生理参数以及DAD的影响。结果 :生理条件下 10 -6 mol/LGA、GB使APD50 分别缩短 9.1%和 6.2 % ;APD90 分别缩短 8.4%和 5 .9%。缺血状态下APD明显缩短、APA减小、RP及 0期Vmax减小 (P <0 .0 5～ 0 .0 1)。GA、GB可延缓和减轻缺血引起的上述变化 (P <0 .0 1) ,GA、GB可降低DAD的发生率 (P <0 .0 1)。结论 :银杏内酯A、B有缓解和改善心肌缺血作用 。Objective:To study the anti-arrhythmic and anti-ischemia effect and mechanism ginkgolide A?B(GA?GB).Methods:Standard microelectrode technique recorded transmembrane activity.Delayed after depolarization(DAD)was induced by low potassium Tyro's solution with cesium chloride.Results:Under control condition,the action potential duration(APD)was slightly shortened by GA?GB.APD 50 9.1% and 6.2% were shortened by 10 -6 mol/L GA?GBshortened,APD 90 8.4% and 5.9%(n=8,P<0.01) respectively.During ischemia condition,APD was shortened;APA,RP and V max in 0 phase was decreased(P<0.05～0.01).Perfusion with ischemia solution containing 10 -6 mol/L GA or GB could attenuate the electrophysiological changes(P<0.01).Conclusion:10 -6 mol/L GA or GB could attenuate myocardial ischemia and block the onset of arrhythmia.

关 键 词：银杏内酯A 银杏内酯B 豚鼠 乳头肌细胞 电生理效应 

分 类 号：R285.5[医药卫生—中药学]