白细胞介素-6相关新基因的生物学分析  

Biological analysis of a novel gene related with interleukin-6

作　　者：夏荣[1] 黎燕[2] 凌伦奖[3] 冯建男[2] 兰炯采[1] 沈倍奋[2] 

机构地区：[1]第一军医大学南方医院输血科,广东广州510515 [2]军事医学科学院基础医学研究所,北京100850 [3]中国科学院生物物理研究所,北京100800

出　　处：《第一军医大学学报》2003年第1期34-37,共4页Journal of First Military Medical University

基　　金：国家杰出青年科学基金(39925019)~

摘　　要：目的通过白细胞介素-6(IL-6)相关新基因的生物学分析,研究IL-6的作用机制。方法利用IL-6相关表达序列标签(EST)进行电子克隆获得924 bp全长新基因,后采用RT-PCR方法从IL-6激活的人U937细胞所提的总RNA中钓取,此片断连到pGEM-Teasy质粒上并测序。结果成功钓取了IL-6相关EST电子克隆的全长cDNA基因。结论使用电子克隆技术对于发现新基因有重要的指导意义。Objective To understand the action mechanism of interleukin (IL)-6 through investigation of its related genes. Methods Using electronic cloning with IL-6-related expressed sequence tags (ESTs), a novel gene at its full length of 924 bp was acquired. Reverse transcriptase-PCR method was subsequently employed to amplify the cDNA of this gene from the total RNA of human U937 cells, which were previously activated with 100 ng/ml IL-6 for 8 h. The fragment was then ligated into the pGEM-Teasy vector and sequence analysis was conducted. Result The whole cDNA gene was successfully fished. Conclusion Electronic cloning technology can be instrumental in identifying novel genes.

关 键 词：白细胞介素-6 相关新基因 生物学分析 

分 类 号：R392[医药卫生—免疫学]