硝酸纤维素膜在细胞培养和染色中的应用  

Application of nitrocellulose membrane in cell culture and pathological techniques

作　　者：张泓[1] 王亚东[2] 赖卓胜[2] 

机构地区：[1]第一军医大学南方医院外科实验室,广东广州510515 [2]第一军医大学南方医院全军消化病研究所,广东广州510515

出　　处：《第一军医大学学报》2003年第1期89-90,共2页Journal of First Military Medical University

摘　　要：目的提供一种新的用于细胞培养和病理技术的支持介质。方法应用硝酸纤维素膜培养细胞和病理染色并与常规方法培养细胞的比较。结果细胞生长良好,形态正常,染色后结构清晰,与常规玻璃培养的细胞无明显差异。结论硝酸纤维素膜具有良好的透光性及化学稳定性,无细胞毒性作用,同时对细胞有较好的亲合性,可以替代玻片,较玻片具有广阔的应用前景。Objective To provide a new supporting medium for carrying out cell culture and histological staining. Methods LoVo cell line was cultured using nitrocellulose membrane as the culture medium, in parallel with the same cell culture on the coverglass which served as control. Results The cells cultured on nitrocellulose membrane showed no visible difference from those cultured on coverglasses in terms of cell growth, morphology, and the structure displayed after staining. No toxic effect was observed due to the application of nitrocellulose membrane. Conclusions Nitrocellulose membrane after treatment has such merit as good lucidity and stability without toxicity on the cells. Having also good affinity with the cells, nitrocellulose membrane can offer an promising alternative for glass as cell culture medium.

关 键 词：硝酸纤维素膜 细胞培养 应用 病理染色 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]