垂体腺苷酸环化酶激活肽对家兔实验性动脉粥样硬化血管重塑的影响  

Effects of Pituitary Adenylate Cyclase Acfivating Pepypeptide on Vascular Remodeling in Experimental Atherosclerotic Rabbits

作　　者：任彩霞[1] 常青[1] 李自成[2] 李建芝[1] 唐海兰[1] 黄华梅[1] 关洁宾[3] 

机构地区：[1]暨南大学医学院组织学与胚胎学教研室,广州510632 [2]暨南大学医学院附属第一医院心内科,广州510630 [3]暨南大学医学院动物所,广州510632

出　　处：《中国动脉硬化杂志》2002年第6期465-468,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金项目 (3 980 0 0 68)资助

摘　　要：为探讨垂体腺苷酸环化酶激活肽对家兔动脉粥样硬化血管重塑的影响 ,将 6 0只新西兰雄性家兔随机等分为正常对照组、动脉粥样硬化组和垂体腺苷酸环化酶激活肽组 ,分别于实验的第 4、8和第 12周末随机处死每组家兔各 5～ 8只并取胸主动脉。在上述动脉条的头、中、尾部各截取约 0 .5cm长的组织作石蜡切片 ,苏木素—伊红染色 ,光镜下观察并用图像分析系统测量相关形态学参数。余下的动脉条用苏丹Ⅳ染色 ,作大体形态观察。结果发现 ,随时间延长 ,动脉粥样硬化组斑块面积逐渐增大 ,斑块检出率逐渐增加 (P <0 .0 5 ) ,且斑块多分布于头段 ,而中段、尾段依次减少。管腔面积在早期斑块形成时并无改变 ,晚期则明显缩小 (P <0 .0 5 )。垂体腺苷酸环化酶激活肽组斑块面积、斑块最大厚度均小于同期动脉粥样硬化组 ,而管腔面积则大于动脉粥样硬化组 (P <0 .0 5 )。Aim To investigate the effects of pituitary adenylate cyclase activating polypeptide (PACAP) on vascular remodeling in atherosclerotic rabbits. Methods 60 male New Zealand rabbits were divided randomly and equally into three groups: control group,atherosclerosis (As) group and PACAP group. 5～8 rabbits of each group were sacrificed at the end of the fourth,eighth and twelfth week and the thoracaortae were collected. The segments about 0.5 cm long from the beginning, middle and end part of the above aortae were obtained to be made into paraffin sections and stained with hematoxylin eosin(HE), then the sections were observed by light microscope and the related parameters were measured by image analysis system. The rest part of the aortae were stained with Sudan Ⅳ and surveyed by naked eyes. Results Plaque area and percentage of plaque formation increased gradually with the proceeding of experiment in As group (P<0.05), and plaque mostly located in the beginning segment, decreased in the middle and end segments in turn. Lumen area varied rarely in early plaque formation, while decreased significantly at the advanced stages (P<0.05). Plaque area and maximal plaque thickness in PACAP group were smaller than those in As group, but lumen area was larger than that in As group(P<0.05). Conclusion PACAP can retard the progression of As by inhibiting negative remodeling and promoting positive remodeling.

关 键 词：垂体腺苷酸环化酶激活肽 动脉粥样硬化 血管重塑 

分 类 号：R543.5[医药卫生—心血管疾病]