血管内皮生长因子与家兔动脉粥样硬化病变进展的关系  

Relationship between Vascular Endothelial Growth Factor and the Pathogenesis of the Experimental Atherosclerosis of Rabbits

作　　者：李建芝[1] 常青[1] 唐海兰[1] 黄华梅[1] 关洁宾[2] 李自成[3] 

机构地区：[1]暨南大学医学院组织学与胚胎学教研室,广州510632 [2]暨南大学医学院实验动物所,广州510632 [3]暨南大学医学院附属第一医院心内科,广东省广州市510630

出　　处：《中国动脉硬化杂志》2002年第6期476-478,共3页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(3 980 0 0 68)资助

摘　　要：为探讨血管内皮生长因子与动脉粥样硬化病变进展的关系。将 5 0只雄性新西兰家兔随机分为二组 :对照组动物喂饲普通颗粒兔饲料 ;动脉粥样硬化模型组动物喂饲含胆固醇 (每只 1.5g d)颗粒兔饲料。分别于实验的第 0、4、8、12周末每组处死兔各 5～ 8只 ,取主动脉 ,采用宏观和微观相结合的方法 ,定性及定量观察病变进展情况。同时用免疫组织化学SP法观察血管内皮生长因子的表达。大体标本及显微标本定性观察均发现 ,随时间延长 ,动脉粥样硬化组兔主动脉的斑块面积逐渐增大 ,斑块厚度增加。显微图象定量测量的结果与定性观察结果一致 ,12周末时 ,斑块平均厚度为 5 .78± 3.75 μm ,明显大于 4周末的 1.2 8± 0 .86 μm ,两者相比 ,差异显著 (P <0 .0 5 ) ,斑块最大厚度可达 2 6 .16 μm。残存管腔面积呈现逐渐狭窄的趋势。免疫组织化学染色发现 ,正常兔主动脉组织中无血管内皮生长因子表达 ,而动脉粥样硬化斑块区内血管内皮生长因子表达呈阳性 ,且随动脉粥样硬化病变进展 ,血管内皮生长因子表达逐渐增强 ,阳性区域的面积也逐渐增大。此结果提示 ,血管内皮生长因子在动脉粥样硬化的发生发展中可能发挥着重要的促进作用。Aim To investigate the relationship between vascular endothelial growth factor (VEGF) and the development of atherosclerosis (As). Methods 50 male rabbits were divided into two groups: the rabbits in control group received normal feed, each rabbit in As group received the feed with 1.5 g cholesterol every day. The aortas of rabbits were harvested and observed the pathological changes at the end of week 0, week 4, week 8, and week 12. VEGF expression was studied with immunohistochemical techniques in these samples. Results The results of qualitative observation and quantitative measure are coincident. The average plaque thickness, maximal plaque thickness of aortae in As group gradually increased. The percentage of residual luminal area in As group appears the trend of narrowing gradually. At 12th weekend, the average plaque thickness (5.78±3.75 μm) is significantly larger than that at 4th weekend (1.28±0.86 μm)( P<0.05), the maximal plaque thickness reach 26.16μm. Normal arterial segments showed no VEGF expression, while atheromatous lesions showed distinct VEGF expression. Furthermore, VEGF positivity was intensified gradually as the pathology developed. Conclusion VEGF may have some role in the progression of atherosclerosis.

关 键 词：血管内皮生长因子 动脉粥样硬化 心血管疾病模型 

分 类 号：R543.5[医药卫生—心血管疾病]