苦瓜蛋白对BALB/C小鼠柯萨奇B3病毒性心肌炎核因子κB的调节作用  

Regulation Effect of Momordicin to Nuclear Factor Kappa B Level of CVB3 Viral Myocarditis BALB/C Model

作　　者：王佐[1] 李双杰[2] 杨永宗[3] 

机构地区：[1]中南大学基础医学院,湖南省长沙市410078 [2]南华大学附属第一医院,湖南省衡阳市421001 [3]南华大学心血管病研究所,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2002年第6期479-482,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金 (3 0 0 70 93 4)资助

摘　　要：为探讨苦瓜蛋白对BALB C小鼠柯萨奇B3病毒性心肌炎的治疗作用机制 ,以核因子κB为对象 ,来观察苦瓜蛋白的调节作用。从苦瓜果肉中提取苦瓜蛋白后 ,将动物分为 5组 :病毒对照组、正常对照组、药物对照组、高剂量治疗组、低剂量治疗组 ,分别在实验的第 0d、3d、7d、14d和第 2 1d处死动物 ,提取核蛋白 ,在用BCA方法进行蛋白定量后作凝胶滞留分析 (EMSA) ,测定核因子κB的活性 ,并进行比较分析。结果发现 ,①病毒对照组在第 2 1天时仍然有一定量的核因子κB活化 ,且其活化程度明显高于正常对照组 ;②高、低剂量的苦瓜蛋白对核因子κB表现出不同程度的抑制作用 ,其中高剂量组核因子κB几乎看不到活化 ,低剂量组比病毒对照组核因子κB活化程度明显降低 ;③正常对照组和药物对照组核因子κB只有低水平活化。结果提示 。Aim Our previous result showed that at the concentration of 25 mg/(kg.d), Momordicin has a distict therapeutic effect on coxsackievirus B3 (CVB3) viral myocarditis. Because nuclear factor kappa B (NF κB) is Key tactor in immunity and inflammatory reaction, we measure the regulation effect of Momordicin to the level of NF κB. Method To purified Momordicin from Momordica as our previous report, 5 groups of animal are used in this experiment: control of virus group (VC group); control of normal group (NC group); control of Momordicin group (MC group); low dose Momordicin therapeutic group (LM group); high dose monordicin therapeutic group (HM group). Animals were killed at 0 d, 3 d, 7 d, 14 d and 21 d, and purified nucleus protein, protein quantifyed by BCA method, then measuring the activity of EMSA and assys the result. Result The activity of NF κB are still measuerable in the DC group at 21 d, and it's activity is higher than the NC group; there are different degree of inhibition effect in HM and LM groups to NF κB activity, and nerely no NF κB was appeared in the HM and MC groups; there are low level NF κB acitivity were found in the NC group. Conclusion The curing effect of momordicin to viral myocarditis may related to it's inhibitory effect to NF κB activity.

关 键 词：苦瓜蛋白 病毒性心肌炎 核因子ΚB 心血管疾病模型 

分 类 号：R542.21[医药卫生—心血管疾病]