TSG-6基因在脂肪组织中的表达及其与肥胖的关系  

Identification of tumor necrosis factor alpha-stimulated gene-6 gene and obesity: a new gene expression in adipose tissue

作　　者：丁胜利[1] 郭锡熔[1] 陈荣华[1] 费莉[1] 龚海霞[1] 池霞[1] 倪毓辉[1] 

机构地区：[1]南京医科大学儿科医学研究所,210029

出　　处：《实用儿科临床杂志》2003年第1期14-15,共2页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(项目编号:39870362);江苏省教委自然科学基金(项目编号:98KJB320002)

摘　　要：目的 鉴定TSG-6基因在正常和肥胖儿童脂肪组织中的表达,探讨该基因与肥胖的关系。方法采用RT-PCR技术检测正常和肥胖儿童皮下脂肪组织中TSG-6基因的表达水平,并测序鉴定。结果 TSG-6基因表达于儿童的脂肪组织,且肥胖组患儿皮下脂肪组织中TSG-6基因的表达水平显著高于正常对照组。结论TST-6基因是一个新的与肥胖发生相关基因,可能与肿瘤坏死因子-α(TNF-α)、白细胞介素1(IL-1)共同调节肥胖的发生。Objective To study the differential expressions of tumor necrosis factor alpha-stimulated gene-6(TSG-6) gene in adipose tissue of normal and obese children and discuss the relationship between TSG-6 gene and obesity. Methods Total RNA from the subcutaneous adipose tissue was extracted and the level of TSG-6 gene mRNA expression was evaluated by RT-PCR. Results The TSG-6 gene mRNA expression levels in subcutaneous adipose tissue of obese human beings were significantly higher than those in normal persons. Conclusions TSG-6 gene is a new gene which is involved in adipogenesis. Presurnablyi the pathological changes of obesity may be regulated by TNF-alpha, IL-1 and TSG-6 gene together.

关 键 词：TSG-6基因 脂肪组织 肥胖 

分 类 号：R723[医药卫生—儿科]