毛细管电泳用于评价脱氧核糖核酸退火反应  

Using Capillary Electrophoresis to Evaluate Deoxyribonucleic Acid Annealing Reaction

作　　者：何新亚[1] 周小棉[1] 林炳承[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023

出　　处：《分析化学》2003年第1期10-13,共4页Chinese Journal of Analytical Chemistry

摘　　要：将毛细管电泳技术用于定性和定量评价互补单链脱氧核糖核酸 (DNA)的退火反应 ,从电泳信号能直接观察退火效率。解释了双链DNA的电泳迁移特性 ,对DNA产品基质中的NaCl浓度进行了测定 ,考察了DNA样品中NaCl浓度对进样的稀释效应。在所应用的实验条件下 ,NaCl浓度在 2 0mmol L以内稀释效应的影响是可以忽略的。Capillary Electrophoresis was used to evaluate the annealing of two complemental single stranded deoxyribonucleic acid (DNA) qualitatively and quantitatively. The annealing efficiency can be observed from the electropherogram. The migration characteristic of the double stranded DNA was explained. The NaCl concentration in the annealing DNA product matrix was determined. In general the existence of NaCl can lead the sample to dilute, but this factor could be neglected when the concentration of NaCl, was < 20 mmol/L in our experimental conditions.

关 键 词：评价 退火反应 毛细管电泳 脱氧核糖核酸 DNA 合成 分析 脱盐 

分 类 号：O658.9[理学—分析化学]