反相高效液相色谱法快速测定7种四环素类抗生素  

Rapid Determination of 7 Tetracyclines by Reversed Phase High Performance Liquid Chromatography

作　　者：王蕾[1] 徐智秀[1] 张孝松[1] 邵学广[1] 

机构地区：[1]中国科学技术大学化学系,合肥230026

出　　处：《分析化学》2003年第1期52-54,共3页Chinese Journal of Analytical Chemistry

摘　　要：建立了一种反相高效液相色谱梯度洗脱分离测定四环素类药物的新方法。采用DiamonsilTM C1 8ODS(2 5 0mm× 4 .6mmi.d ,5 μm)色谱柱 ,以甲醇 乙腈 0 .0 1mol/L草酸为流动相 ,流速 1.0mL/min ,2 70nm检测 ,在10min内分离检测四环素等 7种化合物。同时还研究了流动相组成、梯度条件、pH值、草酸浓度等因素对分离效果的影响。A gradient elution method for the determination of 7 tetracyclines (TCs) by reversed phase high performance liquid chromatography (RP-HPLC) was proposed. TCs were separated on a Diamonsil (C-18 column (250 x 4.6 min i.d., 5 mum) using methanol-acetonitrile-0.01 mol/L oxalic acid at a flow rate of 1.0 mL/min and detected at. 270 nm within 10 min. The effects of the distribution proportion of the mobile phase, gradient elution program, pH value and the oxalic acid concentration on separation were discussed.

关 键 词：反相高效液相色谱 四环素类抗生素 紫外检测 梯度洗脱 药物分析 

分 类 号：O657.7[理学—分析化学]