表面等离子体子共振生物传感器用于乙肝表面抗原的测定  

Determination of Hepatitis B Surface Antigen by Surface Plasmon Resonance Biosensor

作　　者：陈泽忠[1] 王柯敏[1] 羊小海[1] 黄杉生[1] 黄红梅[1] 李杜[1] 王青[1] 

机构地区：[1]湖南大学化学化工学院,湖南大学化学生物传感与计量学国家重点实验室,长沙410082

出　　处：《化学学报》2003年第1期137-140,共4页Acta Chimica Sinica

基　　金：国家杰出青年基金(No.2 982 51 1 0 );湖南省自然科学基金(No.0 0GKY1 0 1 0 )资助项目

摘　　要：运用自行研制的表面等离子体子共振(SPR)生物传感器,采用自组装成膜技术并以戊二醛作偶联剂,在传感片表面修饰HBsAg单克隆抗体,将其用于乙肝表面抗原(HBsAg)的检测.实验结果表明SPR生物传感器对HBsAg的检出限为 0.0 6ng/mL.与传统的酶联免疫吸附试验(ELISA)相比,SPR生物传感器的检出灵敏度明显高于ELISA法.用该SPR生物传感器对HBsAg质控血清与纯化的HBsAg溶液进行比较检测。A spectral surface plasmon resonance (SPR) biosensor has been used to determine the hepatitis B surface antigen (HBsAg). The anti-HBsAg monoclonal antibody was immobilized on the surface of the sensor chip using the technology of self-assembly and a bifunctional cross-linker. The detection limit of HBsAg solution was 0.06 ng/mL. The results of responses to HBsAg control serum and to pure HBsAg solution showed that the selectivity of SPR biosensor was good. The results of experiments showed that the detection limit of the SPR biosensor was much lower than that of enzyme-linked immuosorbent assay (ELISA) method.

关 键 词：测定 表面等离子体子共振 生物传感器 表面抗原 自组装膜 乙型肝炎 诊断 HBSAG 

分 类 号：R318.6[医药卫生—生物医学工程]