新型天然血管支架:猪胸主动脉脱细胞血管基质的制备  

Methods of preparing porcine thoracic aortas acellular matrix the new nature scaffold of vascular

作　　者：韩雪峰[1] 杨大平[1] 郭铁芳[1] 徐学武[1] 张颖[1] 

机构地区：[1]哈尔滨医科大学附属第二医院,黑龙江哈尔滨1500086

出　　处：《中国临床康复》2002年第24期3696-3696,3699,T004,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(30170970);黑龙江省自然基金资助项目(D-0021)

摘　　要：目的研究用酶-化学除垢剂联合法制备猪胸主动脉脱细胞血管。方法猪的新鲜胸主动脉经消毒后,于离心管中经过胰蛋白酶+EDTA,曲拉通X-100溶液作用后取标本。血管体积与各作用溶液体积比为3∶7。标本作苏木素—伊红、弹力纤维染色,大体、光镜及扫描电镜观察。结果胸主动脉中的细胞成分被除去,留下成分主要包括胶原纤维、弹性蛋白等。结论酶-化学除垢剂联合法可将猪胸主动脉细胞成分除去。胰蛋白酶,曲拉通X-100是制备血管脱细胞基质(ACTM)的良好试剂。Objective Remove all the cells from porcine thoracic aortas by enzymatic-detergen t extraction procedure.Methods Fresh thoracic aortas were put into centrifugal tubes with the solution of tryp sin +EDTA.for 24h.After that,aorta s were immerged into the solution of Triton X-100.The specimens were d one with haematoxylin -eosin stain a nd elastin stain,observed grossly,in the light and scanning electron microscopy.Results All the cells have been removed and th e acellular matrix was composed of in soluble collagen,elastin,and some insoluble metamorphic organelles.Conclusions The multistep enzymatic-detergent extraction procedure removes all th e cells from tissues successfully.Trypsin and Triton X-100are good reagents in preparing th e porcine thoracic aortas acellular matrix.

关 键 词：脱细胞基质 血管 胰蛋白酶 曲拉通X-100 

分 类 号：R381.11[医药卫生—医学寄生虫学]