血管内皮细胞与聚羟基丁酯细胞相容性的实验研究  

Cytocompatibility studies on polyhydroxybutyrate combined with blood vessel endothelial cell in vitro

作　　者：袁文周[1] 艾玉峰[1] 熊猛[1] 夏文森[1] 张琳西[1] 董兆麟[2] 杨清芳[3] 

机构地区：[1]解放军第四军医大学西京医院,陕西西安710032 [2]西北大学,陕西西安710068 [3]西北工业大学,陕西西安710072

出　　处：《中国临床康复》2002年第24期3698-3699,T005,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(30070777)

摘　　要：目的评价新型可降解生物材料聚羟基丁酯(PHB)与血管内皮细胞的细胞相容性。方法采用培养的牛血管内皮细胞接种在PHB膜片及胶原包埋的PHB膜片上,并与PGLA和空白对照作比较,用相差显微镜和扫描电镜观察细胞的粘附和生长情况,并对细胞增殖用MTT法进行测定。结果牛血管内皮细胞在四个实验组中粘附良好,生长符合其生长曲线,胶原包埋PHB较单纯PHB细胞更易粘附生长,较PGLA略差。结论牛血管内皮细胞与PHB细胞相容性好,其用胶原包埋可增加细胞亲和性。Objective To study the cytocompatibility on Polyhydroxybutyrate combined with bl ood vessel endothelial cell(BVECs )in vessel tissue engineering.Methods BVECs were cultured with PHB and PHB c oated with collagen,compared with P GLA and control,the morpho-logical characters and cell prolife ration were detected.Result BVECs could be attached to and extended on all materials,PHB coated with collagen was better than PHB,and less than PGLA and control.Conculusion The results show that PHB has good cytocompatibility with BVECs.

关 键 词：血管内皮细胞 聚羟基丁酯 细胞相容性 

分 类 号：R318.11[医药卫生—生物医学工程]