非生物标本的电镜样品制备及超切技术的探讨  

The study of non-biological specimen preparation and ultramicrotomy for election microscopy

作　　者：沈伟[1] 杨海贤[1] 管云林[2] 李治[2] 郭小红[1] 于学宽[1] 赵刚[1] 曾嘉[1] 

机构地区：[1]天津医科大学超微病理教研室,天津300070 [2]天津大学材料学院

出　　处：《天津医科大学学报》2002年第4期411-412,415,共3页Journal of Tianjin Medical University

基　　金：国家自然科学基金资助课题 (编号 :59773002)

摘　　要：目的 :探讨非生物标本的电镜样品制备及超薄切片技术。方法 :选择无色透明纤维、黑色纤维及无色透明薄膜等材料 ,进行脱水、环氧树脂包埋、超薄切片。结果 :切出满意切片 ,并在电镜下拍摄出高质量的照片。结论 :根据非生物标本的特性成功地进行了电镜样品制备及超薄切片 。Objective: To investigate the technology in preparation and ultramicrotomy of non-biological specimens for electron microscopy.Methods:Dehydration,epon embedding and ultra-thin sectioning were carried out with black fibers, colorless hyaloid fibers and membranes.Results:Good quality ultra-thin sections and fine electron micrographs were produced. Conclusion:The preparation of non-biological specimens and their ultramicrotomy were successfully carried out according to their characteristics,which made it possible to study the ultrastructure of non-biological specimens.

关 键 词：制备 非生物标本 超薄切片 电子显微镜 

分 类 号：Q－336[生物学]