肿瘤坏死因子α在肥胖者脂肪组织中的异常表达  

The abnormal expression of tumor necrosis factor-α in the adipose tissue of obesity

作　　者：朱立荣[1] 刘德敏[1] 卢敏[1] 张沫[1] 张捷[1] 

机构地区：[1]天津医科大学代谢病医院,天津300070

出　　处：《天津医科大学学报》2002年第4期429-431,共3页Journal of Tianjin Medical University

基　　金：天津市卫生局科研资助项目 (编号:98KY -41)

摘　　要：目的 :检测肿瘤坏死因子α在肥胖与体重指数正常者腹部皮下脂肪组织中的表达水平 ,观察检测肿瘤坏死因子α与肥胖的关系。方法 :本实验以体重指数>25的个体为实验组 ,体重指数<25的个体为对照组 ,用双抗体夹心ELISA法检测腹部皮下脂肪组织中肿瘤坏死因子α的表达水平。结果 :肿瘤坏死因子α在肥胖者的脂肪组织中表达比对照明显增加 (43 07±12 04与23 12±9 10pg/mg脂肪组织 ,P<0.01) ,并与体重指数呈正相关。结论 :肿瘤坏死因子α与肥胖有关 。Objective: To observe the expression level of tumor necrosis factor-α (TNF-α) in adipose tissue of obesity and to observe the relation of TNF-α and obesity.Methods:Cases with body mass index(BMI) >25 kg/m2 and BMI<25 kg/m2 were divided into experiment group and control group respectively.The expression of TNF-α in adipose tissue was tested with enzyme-linked immune sorbent assay(ELISA).Results:TNF-α level of adipose tissue in obesity group is higher than that in control group(43.07± 12.04 vs 23.12±9.10 pg/mg adipose tissue,P<0.01), and there's a strong positive correlation between the expression of TNF-α and BMI.Conclusion: TNF-α is related to obesity, and expresses higher in adipose tissue of obesity.

关 键 词：表达 肿瘤坏死因子Α TNF-Α 肥胖 脂肪组织 相关性 双抗体夹心ELISA法 

分 类 号：R589.2[医药卫生—内分泌]