高效液相色谱法测定血浆中的阿替洛尔浓度  

Determination of Atenolol in plasma by HPLC

作　　者：焦建杰[1] 张京玲[1] 娄建石[1] 董伟林[2] 张才丽[1] 

机构地区：[1]天津医科大学药理学教研室,天津300070 [2]天津医科大学实验中心

出　　处：《天津医科大学学报》2002年第4期437-439,共3页Journal of Tianjin Medical University

摘　　要：目的 :建立测定人血浆中阿替洛尔浓度的HPLC法。方法 :采用混合有机试剂 (氯仿∶异丙醇 ,75∶25,V/V)萃取样品中的药物 ,对干燥后的残渣用流动相复溶 ,应用高效液相色谱法分离 ,采用荧光检测器检测 (检测波长Ex=225nm ,Em=300nm)。流动相为乙腈∶水∶1mol/L磷酸缓冲液6∶89∶5(V/V/V) ,pH=4 ,流速1ml/min ,内标为美托洛尔。结果 :血浆中阿替洛尔在15.6～1000.0ng/ml范围内 ,药物浓度与峰面积比值呈良好的线性关系 (r=0.9996 ,RUN=3)。总回收率为98.78 %±7.96 %。日内和日间差异均小于10%。最低检出限为10ng/ml。结论 :用改进后的高效液相色谱法测定人血浆中阿替洛尔的浓度具有对样品纯化程度高、分析周期短等优点 。Objective: To establish the way of high performance liquid chromatographic(HPLC) assay for determination of atenolol in human plasma.Methods: Following extraction with a mixture of chloroform isopropanol(75∶25,V/V),the organic phase was evaporated to dryness.The dried extract was reconstituted in 100 μl mobile phase, then separated and determined by HPLC/fluorescence detection Ex=225 nm?Em=300 nm.Results: The calibration curve was linear between 15.6～1000.0 ng/mL with r=0.9996, and the limit detection was 10 ng/mL.The average recovery was 98.78%±7.96%.Intra day and inter day coefficients of Variation were less than 10%.Conclusion: This study provides a simple,accurate and reliable method for pharmacokinetic studies as well as determining concentration of atenolol in human plasma.

关 键 词：测定 高效液相色谱法 阿替洛尔 美托洛尔 荧光检测器 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R972.[医药卫生—药学]