法氏囊三肽囊素在鸭体中的组织学定位及功能的研究  

Histological Location and Effects of Bursin in the Duck

作　　者：谌南辉[1] 郭松林[1] 吴石金[2] 

机构地区：[1]江西农业大学动物科学技术学院,南昌330045 [2]浙江工业大学生物与环境工程学院,杭州310032

出　　处：《畜牧兽医学报》2003年第1期77-81,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(39460060);江西省自然科学基金资助项目(9930015)。

摘　　要：以抗法氏囊三肽素(Bursin)单抗,采用免疫组化法及个体发生早期以该单抗阻抑Bursin效应,检测Bursin在鸭体定位分布及其受阻抑后所致生物学效应。结果表明,BF髓质内周缘呈现阳性细胞层、髓质中央部分有稀少阳性细胞、FAE及FAESE呈阳性、IFSE中阳性细胞散在性呈团簇状分布;Th中阳性细胞相对集中于髓质中类似Hassall小体的区域或较高密度弥散于髓质中;Sp中阳性细胞弥散于GC中或无特征性散在性分布;HG中阳性细胞呈团块状散在分布于腺泡、腺管基底膜中;LN则阳性细胞特征性存在于淋巴小结中,少数散在淋巴细胞聚集区或淋巴组织索中。不同剂量抗Bursin单抗对鸭体细胞免疫功能有较强阻抑效应,高浓度单抗阻抑作用更显著(P<0 01);对体液免疫功能亦有较强阻抑效应,不同剂量单抗间未见显著差异(P>0 05);对BF指数有较强影响,高剂量组更明显(P<0 05),且高剂量对Sp指数有一定影响;较强地抑制BF滤泡发育,高剂量组BF发育更不良。By immunohistochemistry and early immune antagonism with antiBursin monoclonal antibody (McAb)2F94 respectively,the location of Bursin and biological effect of the McAb was studied.The results indicated that inside edge of medulla of BF had a layer of positive McAb cells and middle of medulla had a few positive cells.In addition,FAE and FAESE had positive cells and clusters of positive cells diffused in IFSE of BF;positive cells,in Th gathered in the Hassalllike area or diffused in high density in medulla;positive cells in Sp diffused in GC or dispersed irregular;positive cells in HG located in basement membrane of alveoli and gland duct like conglomeration;positive cells in LN existed in lymphatic nodules characteristically and minority of positive cells spread in mass of lymphoid cells or lymphatic tissue cords.In addition,different dose of antiBursin McAb had comparatively strong antagonistic effect on cellular immune function and hige dose of McAb was more striking (P<001);antiBursin McAb also had comparatively stronger inhibition effect on humoral immune function,but the inhibition effect dosen′t respond to the various dosages of McAb(P>005);high dose of antiBursin McAb had distinct effect(P<005)on index of Fabricii and some influence on index of spleen.There was striking influence(P<005) on early development of BF follicular when injected with high dose of McAb.

关 键 词：法氏囊三肽囊素 鸭体 组织学定位 功能 

分 类 号：S852.4[农业科学—基础兽医学] S834.1[农业科学—兽医学]