一株硝化杆菌的筛选及初步鉴定  

Preliminary Screen and Identification of One Nitrobacteria

作　　者：郑金来[1] 李君文[1] 晁福寰[1] 王新为[1] 周娟[1] 古长庆[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《环境与健康杂志》2003年第1期19-20,共2页Journal of Environment and Health

基　　金：国家863高科技产业发展规划项目(2001AA214191);天津市重大科技攻关项目(0231807111)

摘　　要：目的筛选、分离能够用于治理氮污染的硝化菌。方法通过富集培养从土壤中筛选自养型硝化菌,在进行形态学和生化的检验之后设计引物,扩增硝化菌的特征性基因norB之后对PCR产物进行测序,并与Genbank上的序列比较同源性。结果从土壤中筛选到了1株能够氧化亚硝酸盐的细菌,该菌形态小、浅黄色菌落,是杆菌。PCR能扩增出预期大小片段。对其PCR产物直接测序,与Genbank上的序列比较,发现该基因与硝化菌Nhamburgensis的norB基因同源性高达99%。结论可以初步断定该研究所筛选到的细菌属于硝化杆菌。Objective To screen and seperate nitrobacteria that could be used to treat nitrogenous compound pollution.Methods Autotrophic nitrite-oxidizing microorganisms were screened by enrichment culture from soil.After morphological and biological detection,primers were designed to detect the distinctive gene norB of nitrobacteria.The PCR production was se-quenced,and the homology was identified according to the sequence in Genbank.Results One strain of bacterium was screened that could oxidize nitrite.This bacterium was small,light-yellow bacilli.The expected-size DNA fragments could be amplicated by PCR.The sequence of a PCR production was blasted in Genbank and showed99%consistent with norB gene of N hamburgensis.Conclusion It was preliminarily confirmed that the microorganism screened in this study might be nitrobac-terium.

关 键 词：一株硝化杆菌 筛选 鉴定 硝化杆菌属 聚合酶链反应 基因表达微序列分析 微生物 

分 类 号：R117[医药卫生—公共卫生与预防医学]