高效液相色谱法和微生物法测定人血浆中哌拉西林浓度的比较  

Comparison between high performance liquid chromatography and microbiological assay for the determination of piperacillin in human plasma

作　　者：唐世新[1] 李珍[1] 褚志杰[2] 计一平[1] 范国荣[1] 胡晋红[1] 

机构地区：[1]第二军医大学长海医院药学部,上海200433 [2]武警山东总队医院药剂科,济南250101

出　　处：《药学服务与研究》2002年第4期231-233,共3页Pharmaceutical Care and Research

摘　　要：目的 :比较 HPL C法和微生物法测定血浆中哌拉西林浓度的差异。方法 :6名健康志愿者单剂量静脉滴注哌拉西林 4g,分别用 HPL C法和微生物法测定血浆中药物浓度。 结果 :HPL C法线性范围为 0 .5～ 5 0 0μg/ m l,回收率为 97.40 %～10 0 .0 6 % ,日内、日间 RSD分别为 2 .5 8%～ 3.2 4%和 3.32 %～ 5 .5 9% ;微生物法线性范围为 0 .5～ 10μg/ ml,回收率为98.38%～ 10 6 .85 % ,日内、日间 RSD分别为 3.90 %～ 6 .37%和 7.0 7%～ 9.78%。 结论 :两种方法回收率和精密度均符合要求 ,受试者各时间点平均血药浓度无显著性差异。Objective: To compare the high performance liquid chromatography (HPLC) and microbiological assay for determination of piperacillin in human plasma. Methods: Six healthy volunteers were given a single dose of piperacillin 4 g by infusion. The methods of HPLC and microbiological assay were used to determine the piperacillin plasma concentrations, respectively. Results: The calibration curve of HPLC method was linear in the range of 0.5-500 μg/ml. The recovery rate of HPLC method was 97.40%-100.06%, within-day and day-to-day RSD were 2.58%-3.24% and 3.32%-5.59%, respec-tively. The calibration curve of microbiological assay was linear in the range of 0.5-10 μg/ml. The recovery rate of microbiological assay was 98.38%-106.85%,within-day and day-to-day RSD were 3.90%-6.37% and 7.07%-9.78%,respectively. Conclusion: The recovery rate and the precision of 2 methods are found to be similar. There is no significant difference in piperacillin plasma concentrations measured by HPLC method and microbiological assay.

关 键 词：哌拉西林 高压液相色谱法 微生物法 

分 类 号：R978.11[医药卫生—药品]