HIV感染者和AIDS患者外周血T淋巴细胞亚群的变化  

Peripheral lymphocyte subset alteration in HIV/AIDS patients

作　　者：任莉莉 李富荣 戴勇 齐晖 

机构地区：[1]暨南大学医学院第二附属医院广东省深圳市人民医院临床医学研究中心,518020

出　　处：《广东医学》2003年第1期25-26,共2页Guangdong Medical Journal

摘　　要：目的　通过对艾滋病患者和HIV感染者外周血T淋巴细胞亚群的表达和绝对计数检测 ,研究细胞免疫情况。方法　利用白细胞分类计算和流式细胞分析法对 15例HIV感染者和 2 6例AIDS患者外周血淋巴细胞中CD3 + ,CD4+ ,CD8+ 的表达及绝对数进行检测。结果　AIDS患者组CD3 + 淋巴细胞数较正常值显著下降 (P <0 0 5 ) ;HIV感染组与艾滋病患者组的CD4+ T淋巴细胞数较正常组显著下降 (P <0 0 1) ;CD8+ T淋巴细胞数显著增高 (P <0 0 1) ;CD4+ /CD8+ 的比值显著倒置。结论　人类感染HIV后 ,外周血T淋巴细胞亚群发生显著变化 ,CD4+ 细胞绝对数和CD4+ /CD8+ 比值对于HIV感染的临床进展可以起到指示作用。Objective To study the immune state of HIV/AIDS patients by measuring peripheral lymphocyte subset counts.Methods After white cell count, expression of CD3 +, CD4 + and CD8 + in lymphocytes was investigated by immunofluorescence staining method.Results CD3 +count in AIDS patients was lower than that of healthy controls ( P <0.05). HIV/AIDS patients had lower CD4 + and higher CD8 + than those of normal controls.The CD4 +/CD8 + ratio was significantly inversed. Conclusion After the infection of HIV, peripheral lymphocyte subsets had significantly changed. The absolute count of CD4 + and the ratio of CD4 +/CD8 + can be used as a marker of disease progression.

关 键 词：HIV感染 AIDS 获得性免疫缺陷综合征 淋巴细胞亚群 流式细胞分析法 人免疫缺陷病毒感染 

分 类 号：R512.91[医药卫生—内科学]