浙江蝮蛇蛇毒蛋白水解酶(蛋白酶a)的酶学性质及生物学活性研究  

ENZYMATIC PROPERTIES AND BIOLOGICAL ACTIVITIES OF A PROTEOLYTIC ENZYME (PROTEINASE A) FROM THE VENOM OF Agkistrodon Halys PALLAS

作　　者：张东蕾 王起振[1] 陈建智[1] 

机构地区：[1]沈阳药学院生化教研室

出　　处：《Zoological Research》1992年第4期381-385,共5页动物学研究（英文）

摘　　要：应用离子交换及凝胶柱层析,从浙江蝮蛇(Agkistrodon halys Pallas)蛇毒中分离纯化出一种具有酪蛋白水解活性的蛋白酶a。经研究表明,以酪蛋白为底物,该蛋白酶a作用的最适pH值为9.5,最适温度为45℃,Km值为1.33×10^(-5)mol/L,酶活性受EDTA抑制。蛋白酶a不水解TAME,BAEE等精氨酸脂。实验表明,蛋白酶a具有纤维蛋白溶解作用而无出血毒性,EDTA和半胱氨酸可抑制其纤溶活性。A proteolytic enzyme from the venom of Agkistrodan halys Pallas has been purified by ion-exchang chromatography and by gel-filtration. This proteolytic enzyme (called proteinase a) can hydrolyze casein. Using casein as the substrate, the optimum pH is about 9.5, the optimum temperature is about 45℃,and Km=1.33×10-5 mol/L. Its activity toward casein was inhibited by EDTA. It did not act on TAME and BAEE. Proteinase a showed fibrinolytic activity in vitro, EDTA and cysteine could inhibite this activity.

关 键 词：蝮蛇 蛇毒 蛋白酶 

分 类 号：Q959.620.6[生物学—动物学]