长牡蛎非整倍体的制备、胚胎发育及存活能力  

Embryo development and survival of aneuploid Pacific oyster Crassostrea gigas produced by mating its triploids and diploids

作　　者：巩宁[1] 张国范[1] 

机构地区：[1]中国科学院海洋研究所

出　　处：《中国水产科学》2003年第1期5-9,共5页Journal of Fishery Sciences of China

基　　金：国家自然科学基金资助项目(39970590);国家杰出青年基金资助项目(39825121).

摘　　要：通过二倍体、三倍体杂交的方法制备长牡蛎(Crassostreagigas)非整倍体。实验组设为2n♀×3n♂组、3n♀×2n♂组和3n×3n组,对照组为2n×2n组。与对照组相比,实验组畸形率高,担轮虫孵化率及D形幼虫生成率等参数均降低,幼虫死亡率高。在担轮幼虫向D形幼虫转化期,实验组发育滞后。培育过程中染色体数为25左右的非整倍体不能存活,整倍体和染色体数与整倍体接近的非整倍体能够存活。实验证实该方法是进行贝类非整倍体制备的有效手段。The aneuploids Pacific oyster Crassostrea gigas were produced by 2n♀×3n♂ (DT group), 3n♀×2n♂(TD group) 3n×3n (TT group). The control group was 2n×2n (DD group) and Compared to the DD group, the other three groups had more abnormal embryos and their embryo development delayed much more during the Dstage. The embryos with 25 chromosomes couldn't survive during the cultivation, but only got 2n larvae peak in number at fifth day. For the DT group, 2.5n larvae dominated when tested at 12th hour and 30th hour after the fertilization. For the TD group, 2.5n larvae got to the peak in number in 12 h after the fertilization, and 30 h later, 2n and 3n larvae both got to their peaks. The results show that the way of aneuploid producing used in this experiment is reliable.

关 键 词：长牡蛎 非整倍体 制备 胚胎发育 存活能力 形成机制 

分 类 号：Q959.215.3[生物学—动物学]