褐藻酸降解菌的筛选及其生长条件  

Screening of alginic acid decomposing bacteria and its culture conditions

作　　者：王艳玲[1] 唐学玺[1] 杨震[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国水产科学》2003年第1期51-54,共4页Journal of Fishery Sciences of China

基　　金：国家重点基础研究专项经费资助(G1999012004).

摘　　要：采用安藤芳明选择性培养基从自然发病明显的海带幼苗上分离出8株褐藻酸降解菌。研究表明,8株菌能够不同程度地降解褐藻酸钠,菌株A1和A2表现出较强的降解能力,接种1d后液体培养基即变清。温度是该菌大量繁殖的决定性因子,其生长的最适温度为20℃,最适pH7.5。褐藻酸钠质量分数为0.5%～0.6%时,不同氮源和碳源对菌体生长的影响不同,其中有机氮和铵态氮有利于菌体生长,尿素和硝酸钠抑制菌体生长;只有在培养基中含有褐藻酸钠时,菌体才能正常生长。Eight strains of alginic acid decomposing bacteria were isolated from decaying thalli Laminaria and all the strains had the ability to degrade sodium alginate in varying degrees. Among them two strains (A1 and A2) showed higher degrading intensity that the culture medium became clear only one day after the inoculation. The main affecting factor for the reproduction of the strains was temperature. The bacteria had a maximum biomass when the liquid media contained 0.5% sodium alginate and 0.5% organic nitrogen, pH 7.5 and temperature 20 ℃ after 72 h incubation.

关 键 词：褐藻酸降解菌 生长条件 海带 腐烂病 筛选 

分 类 号：S946.11[农业科学—水产养殖]