东方铃蟾不同发育阶段乳酸脱氢酶同工酶的变化  

CHANGES OF ISOZYME PATTERNS OF LACTATE DEHYDROGENASE DURING THE ONTOGENY OF BOMBINA ORIENTALIS

作　　者：郑元林[1] 陈才法[1] 茅一飞[1] 

机构地区：[1]徐州师范学院生物系,221009

出　　处：《动物学杂志》1992年第4期18-21,共4页Chinese Journal of Zoology

摘　　要：东方铃蟾乳酸脱氢酶(LDH)同工酶的表达在不同发育阶段以及不同的组织有其特异性。心脏中以LDH-1占优势,骨骼肌和肝脏中以LDH-5占优势,脑中LDH-1与LDH-5的相对含量接近。从鳃盖期至成体,心、肝、骨骼肌的LDH同工酶谱型不发生转换。心脏的LDH同工酶含量无阶段间的差异;肝、骨骼肌的LDH同工酶相对含量变化发生在从尾退化期至成体这一时期。在尾退化期,脑中LDH同工酶谱型发生转换,但LDH同工酶相对含量变化的幅度较小。This paper reports the changes of isozyme patterns of lactate dehydro-genase (LDH) during the ontogeny of Bombina orientalis. This investigation clearly demo. nstrates specificityes of LDH isozyme patterns during different ontogeny stages in different tissues. Nearly every tissue, in fact, contains all five isozymes in measurable amounts. LDH-l is more abundant in heart; on the contrary LDH-5 predominates in skeletamuscles and liver; The amounts of LDH-1 and LDH-5 are almost equal in brain. From operculum formation stage to adult there is not any marked shift of isozyme patterns in heart, skeletal muscle and liver, the amount of LDH isozyme does not change between different develo-pment stages in heart tissues. The change of LDH isozyme amount occurs during the period of tail degeneration to adult stage in skeletal muscle and liver, i.e. LDH-5 in adult is more abundance than that in tail degeneration stage. However, the LCH isozyme patterns shift between operculum formation stage and tail degeneration stage, but the relative abundance of LDH isozyme chages in sm醠i amounts. The relative abundance of the different isozymes corresponds with the metabolic requirements of the tissues.

关 键 词：东方铃蟾 乳酸脱氢酶 同功酶 发育 

分 类 号：Q959.530.5[生物学—动物学]