肝郁脾虚因素促进DEN诱发大鼠实验性肝癌的研究  

Research on the Impact of Liver-stagnation and Spleen-deficiency on Experimental Hepatocarcinoma Induced by DEN in Rats

作　　者：王济[1] 顾立刚[1] 王庆国[1] 彭桂英[1] 李瑞生[1] 王秀茜[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中国中医药信息杂志》2003年第1期18-20,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家中医药管理局科研基金资助项目(№.37600176);人事部留学回国人员A类重点课题资助项目(2001)

摘　　要：目的:观察肝郁脾虚因素刺激对二乙基亚硝胺(DEN)诱发大鼠实验性肝癌发生及免疫功能的影响。方法:建立大鼠实验性肝癌肝郁脾虚证动物模型。大鼠给予诱癌剂(DEN70mg/kg灌胃,每周1次,连续8周),同时给予肝郁脾虚(束缚加饮食失节法)刺激至第19周,断头处死,做病理切片,HE染色,镜下观察肝组织病变;取肝组织用流式细胞术检测DNA含量;测定脾脏T、B淋巴细胞转化率和腹腔巨噬细胞吞噬中性红能力。结果:肝郁脾虚因素可明显降低该模型大鼠的体重和免疫功能,并可使肝细胞DNA含量发生异常变化。结论:肝郁脾虚因素可促进DEN诱发大鼠实验性肝癌的发生发展。Objective:To investigate the effect of liver-stagnation and spleen-deficiency on the immue function of rats and the contents of DNA in liver cells in rats of experimental hepatocarcinoma induced by DEN. Methods:Rats were stimulated with the factor of liver-stagnation and spleen-deficiency for 18weeks and simultaneously induced by DEN 70mg/kg once a week for 8 weeks. The pathological changes of liver and contents of DNA in liver cells were detected;T-lymphocyte transformation rate, B-lymphocyte transformation rate of the spleen and phagocytic function of peritoneal macrophage were tested. Results: Body weights of the rats could be decreased, the contents of DNA in liver cells could also be changed and the immune functions could be impaired significantly by stimulation of liver-stagnation and spleen-deficiency. Conclusion:The factor of liver-stagnation and spleen-deficiency could promote the development of experimental hepatocarcinoma induced by DEN in rats.

关 键 词：肝郁脾虚 实验性肝癌 二乙基亚硝胺 免疫功能 中医药疗法 

分 类 号：R273.57[医药卫生—中西医结合]