细胞色素C的快速测定研究  

Study on Quick Determination of Cytochrome C

作　　者：陈月开[1] 黄登宇[1] 强玉丰[1] 王美素[2] 

机构地区：[1]山西大学生命科学与技术学院,山西太原030006 [2]山西中医学院,山西太原030024

出　　处：《山西大学学报（自然科学版）》2003年第1期64-66,共3页Journal of Shanxi University(Natural Science Edition)

摘　　要：用756分光光度计扫描测定细胞色素C,发现在315nm、415nm、520nm和550nm波长处有特征吸收峰;以细胞色素C的不同浓度分别对该四种波长下的光密度值回归,方程均为y∧=a+bx,a、b值各不相同;线性相关系数分别为r315=0.9915、r415=0.9999、r520=0.9835、r550=0.9977;从扫描波谱图形和线性相关系数比较,均以415nm波长效果最好.该法操作简便、快速、用药量小.Regression equations for CytC(Y) and A315,A415,A520 and A550(Z) were set up by using concentration and optical density that were determined with 756 spectrometers,characteristic absorb values of CytC are at 315 nm,415 nm,520 nm and 550 nm.The best linear relation is at 415 nm,so the content of CytC may be confirmed with A415value.It is a very conveniently,quickly method to determine CytC by using 756 spectrometers that only need little dosage,which may be used as a supply determination metnod of CytC in pharmacopoeia.

关 键 词：快速测定 细胞色素C 分光光度计 回归方程 特征吸收峰 活力测定 中药 

分 类 号：R284.1[医药卫生—中药学] Q559.9[医药卫生—中医学]