丙型肝炎病毒包膜蛋白E2人源单链可变区抗体的筛选与鉴定  

SCREENING AND CHARACTERIZATION OF HUMAN PHAGE ANTIBODY TO HEPATITIS C VIRUS E2 ANTIGEN

作　　者：钟彦伟[1] 成军[1] 施双双[1] 王刚[1] 陈菊梅[1] 

机构地区：[1]解放军第302医院传染病研究所基因治疗研究中心,北京100039

出　　处：《解放军医学杂志》2003年第1期53-54,共2页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助项目 (编号C390 0 1 30 )

摘　　要：采用噬菌体表面展示技术 ,以重组的HCV结构蛋白E2为固相包被抗原 ,从半合成的噬菌体单链可变区抗体库中经过 3轮“吸附 洗脱 扩增”筛选过程 ,获得抗原特异性和结合活性较强的HCVE2人源单链可变区抗体 (ScFv)片段 ,片段为 771bp,阳性克隆 ,对其进行免疫学检测 ,并对HCVE2特异性ScFv的编码基因序列进行测定分析。结果筛选得到的HCVE2ScFv片段 (771bp)To screen and characterize human phage antibody to hepatitis C virus E2 antigen. The recombinant phages were panned by HCV E2 antigen which was coated in a microwell plate. After three rounds of biopanning, 56 clones specific to HCV E2 antigen were obtained. The specificity of scFv was determined by ELISA method and cross reaction with BSA and competitive inhibition assay. HCV E2 phage antibody had a specific combination character with recombinant hepatitis C virus E2 antigen. The DNA sequence data showed that the scFv coding gene was 771bp in size

关 键 词：丙型肝炎病毒 包膜蛋白E2 人源单链可变区抗体 筛选 鉴定 

分 类 号：R512.63[医药卫生—内科学] R373.2[医药卫生—临床医学]