大鼠在反复高加速度暴露后脑组织中bcl-2、bax、p53和ICE的表达  

CHANGES IN mRNA EXPRESSIONS OF bcl-2、bax、p53 AND ICE GENE IN CEREBRAL TISSUS OF RATE EXPOSED TO REPEATED+Gz

作　　者：蔡庆[1] 刘红巾[1] 姜树强[1] 姜建东[1] 占志[1] 朱美财[1] 

机构地区：[1]空军总医院,北京100036

出　　处：《解放军医学杂志》2003年第1期81-82,共2页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科研"九五"计划基金 (编号 98D0 33);空军后勤部科研基金(编号KH991 90 76)资助课题

摘　　要：为了解反复高正加速度 (+Gz)暴露后大鼠脑组织中bcl 2、bax、p5 3和白细胞介素 1β转化酶基因的表达变化 ,探讨细胞凋亡在反复高 +Gz暴露所致脑损伤中的病理作用 ,用半定量逆转录聚合酶链反应方法检测反复高 +Gz暴露后大鼠脑组织中bcl 2、bax、p5 3和ICE表达水平 ,并用原位末端脱氧核苷酸转移酶标记法检测脑组织中的凋亡细胞。结果bcl 2在 +Gz重复暴露后 6h和 2 4h表达明显降低 ,而bax、p5 3和ICE在 6h和 2 4h表达明显升高 ,6h组和 2 4h组在大脑皮质、海马CA1区和纹状体可见部分神经元发生凋亡。表明反复高 +Gz暴露可引起大鼠脑组织中bcl 2、bax、p5 3和ICE表达变化 ,细胞凋亡是反复高The purpose of this study is to observe the changes in gene expressions of bcl 2, bax, p53 and interleukin 1β converting enzyme (ICE) in the cerebral tissue of rat exposed to repeated +Gz, and to explore the pathogenetic role of apoptosis in brain damage induced by repeated +Gz exposures. The expression levels of bcl 2, bax, p53 and ICE in cerebral tissue of rats exposed to repeated +Gz were measured by semi quantitative reverse transcription polymerase chain reaction (RT PCR), and the apoptotic cells in brain tissue were detected by terminal deoxynuleotidyl transferase mediated dUTP nick end labeling technique. The results showed that the bcl 2 expression levels in the brain 6h and 24h after repeated +Gz exposures were significantly lower than those of control group, whereas the bax, p53 and ICE expression levels in the brains tissue 6h and 24h after repeated +Gz exposures were significantly higher than those of control group. Apoptotic cells could be observed in cerebral cortex, CA1 subregion of hippocampus and striatum at 6h and 24h after repeated +Gz exposures. It is suggested that the changes in bcl 2, bax, p53 and ICE expressions in rat brain can be induced by repeated +Gz exposures and apoptosis might be one of the molecular mechanisms of brain damage induced by repeated +Gz exposures

关 键 词：大鼠 加速度 脑组织 BCL-2 BAX P53 ICE 基因表达 

分 类 号：R852.21[医药卫生—航空、航天与航海医学]