前列腺素E_2对自体全厚皮片内黑色素细胞增殖规律的影响  

Influence of prostaglandin E_2 on preliferation of melanocytes in full-thickness skin graft

作　　者：李海洋[1] 李武修[1] 孙乐刚[1] 

机构地区：[1]山东滨州医学院附属医院口腔颌面外科,256603

出　　处：《中华整形外科杂志》2003年第1期54-56,共3页Chinese Journal of Plastic Surgery

基　　金：山东省自然科学基金资助 (项目编号 :92C0 72 4)

摘　　要：目的　研究自体全厚皮片前列腺素E2 含量变化对表皮黑色素细胞增殖及密度变化的影响。方法　 6 8只豚鼠随机分为空白对照组、实验 1组 (只作皮片移植 )及实验 2组 (皮片移植 +外用双氯灭痛乳剂 ) ,动态观测皮片组织内前列腺素E2 含量变化 ;借助组织化学及放射自显影技术观察皮片表皮内黑色素细胞的增殖及密度的变化。结果　手术后 1～ 7d ,实验 1组前列腺素E2 含量明显上升 ,30d时再次升高 ,然后恢复正常 ;实验 2组前列腺素含量在术后 2d内明显下降 ,此后表现出与实验 1组相同的趋势 ,但其变化幅度较小。实验 1组MC 3H TdR标记指数在术后 2～ 14d升高 ,30d时出现第二个高峰 ;MC密度在术后 3d内明显下降 ,此后开始升高 ,2 1d后明显超过术前水平 ;实验 2组在术后 1d即开始上升 ,与实验 1组相比 ,出现时间早 ,且变化幅度较小。而实验 2组MC密度同样表现出首先减少然后增加的趋势 ,但是其变化幅度明显小于实验 1组 ;尤为明显的是 ,在实验的最后阶段 ,其MC密度仍然明显低于实验 1组及对照组。结论　在皮片移植早期 ,应用双氯灭痛可以减轻炎症反应 ,并最终抑制黑色素细胞的增殖 ,减少黑色素细胞的密度 ,从而减轻皮片的色素沉着。ObjectiveTo investigate the influence of the prostaglandin E 2 on the proliferation of the melanocytes in the full|thickness skin graft. Methods Sixty|eight guinea|pigs were divided into experimental|1 group (skin graft), experimental|2 group (skin graft+diclofenac), and control groups. After the full|thickness skin graft, the dynamic changes of the prostaglandin E-2 were measured and the preliferation of the melanocyte with its density was also evaluated by using histochemical and autoradiographic methods. Results In the experimental|1 group, the content of PGE 2 was increasing in seven days after the operation, continued to the one month, and then returned to the base level. The labelling indices of 3H-MC|TdR of the group was also increasing postoperatively between the second day and the fourteenth day, and reach a second peak after one month, then came to the normal level. The density of the melanocytes was decreasing rapidly 3 days after the surgery, then began to increase and exceeded over the normal level 21 days after the operation. However, in the experimental|2 group, the content of PGE 2 decreased in two days after the surgery, and then showed the inclination similar to the experimental|1 group with the different points in narrower range. The number of melanocytes labelled by 3H|TdR began to increase at the first day after the surgery, which appeared earlier than the experimental|1 group and was similar in the changing tendency with a less extent. The density of MC showed the similar tendency to the experimental|1 group in a narrower changing range with both of increasing and decreasing. The density of the MC was much lower in 21 after the operation than the experimental|1 group and normal control group. ConclusionThe increased PGE 2 in the earlier stage of the skin grafting could enhance the inflammatory reaction to the tissue, as well as the melanocytes. It may stimulate the preliferation of the MC with the result of increasing their density. The use of the diclofenac might reduce the

关 键 词：自体全厚皮片 细胞增殖 黑色素细胞 前列腺素E2 

分 类 号：R622.1[医药卫生—整形外科]