适于玫烟色拟青霉液相发酵生产的培养基和初始接种量及pH  

Optional medium, initial inoculum and pH control for mass production of the entomopathogenic fungus Paecilomyces fumosoroseus in submerged culture.

作　　者：陈宜涛[1] 冯明光[1] 

机构地区：[1]浙江大学微生物研究所,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2003年第1期39-43,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(39870513)

摘　　要：从美国引进对刺吸式口器害虫具侵染力的玫烟色拟青霉(Paecilomycesfumosoroseus)菌株Pfr116,用5种培养液在25℃下进行深层培养,以菌丝生物量作为指标筛选出了适合于工业生产的发酵液配方(w/v):食用蔗糖2%,马铃薯20%,蛋白胨0.1%,NaNO30.01%,KCl0.01%,MgSO40.025%,MnSO40.005%,FeSO40.005%及KH2PO40.05%。用此培养液对Pfr116菌株进行48h的深层发酵,可获>20mg/mL的干菌丝产量,与萨氏培养液的菌丝产量无显著差异。通过对液培初始接种量和pH值进行优化,确定最佳初始接种量为培养液的菌丝含量1.5～2.0mg/mL,最佳初始pH值为5～7。The entomopathogenic fungal isolate Paecilomyces fumosoroseus Pfr116 highly infective to sucking insects was cultured in submerged conditions at 25 ℃ using five liquid media Based on the yield of dry mycelia over incubation time, a lowcost medium was selected for use in mass production from the five media tested Its components (w/v) included 2% sucrose, 20% potato, and 01% peptone supplemented with 001% NaNO3, 001% KCl, 0025% MgSO4, 0005% MnSO4, 0005% FeSO4 and 005% KH2PO4 A 48h culture of the medium resulted in a mycelial yield of >20 mg/mL, which was close to the mycelial yield of Sabouraud dextrose broth during the same period of incubation Other optional conditions for initiating the culture included: ① the amount of initial inoculum to guarantee the liquid containing mycelial biomass of 1520 mg/mL; and ② a pH ranging from 5 to 7

关 键 词：液相发酵 培养基 初始接种量 PH 玫烟色拟青霉 深层培养 菌丝生物量 昆虫真菌 微生物杀虫剂 

分 类 号：TQ453.5[化学工程—农药化工]