铁蛋白基因的水稻转化及其功能初步分析  

Introduction of ferritin gene into rice and the functional analysis of transgenic plants.

作　　者：徐晓晖[1] 郭泽建[1] 程志强[1] 黎军英[1] 李德葆[1] 

机构地区：[1]浙江大学生物技术研究所,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2003年第1期49-54,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金项目(39980033)资助

摘　　要：铁蛋白是与铁离子结合的一种铁贮藏蛋白,用来调节细胞内的自由铁离子浓度。本研究利用农杆菌介导的转化方法将豌豆铁蛋白(peaferritin,Fer)基因转化到水稻秀水11品种中。经对转基因水稻(T0代)进行了PCR检测,及Southern杂交分析,结果表明,Fer基因已整合到水稻基因组中。通过测定光合作用过程中最大光化学量子产量(Fv/Fm值)分析了由百草枯处理引起的水稻叶片的氧化损害。结果表明,转Fer基因的叶片呈现出不同程度的抗百草枯氧化的能力。用水稻稻瘟病菌接种水稻叶片,发现转Fer基因植株可以减少或延缓病原菌侵染后活性氧的产生。另外,对不同组织中的铁含量测定结果表明,转Fer基因水稻的种子和叶片组织中铁的含量明显提高。这些结果说明Fer在水稻中已表达,增加了转基因植株中铁的贮藏量,同时减少了体内的游离铁从而也减轻了转基因植株的氧化损伤。Ferritin is an iron binding and storage protein. In this study, a pea ferritin (Fer) gene was transferred into rice (Oryza sativa cv Xiushui11, japonica), by Agrobacteriummediated transformation method. The integration of Fer gene into rice genome was confirmed by PCR and Southern blot techniques. Photosynthetic function of the transgenic plants after paraquat treatment was determined by measurement of the maximal yield of the photochemical reaction (Fv/Fm value). The results indicated that transgenic plants exhibited various degrees of tolerance to free radical toxicity. Accumulation of active oxygen species induced by Magnaporthe grisea inoculation was reduced or delayed in transformants as compared with that in the wild type plants. Moreover, tissue iron content of transformants was significantly increased in the seeds and leaves. These results suggest that the expression of Fer gene have elevated tolerance of rice oxidative damage and increased its iron storage, and might provide a promising approach toward overcoming the problem of iron deficient.

关 键 词：水稻 铁蛋白 氧化损害 转基因 农杆菌介导 抗氧化作用 

分 类 号：S511[农业科学—作物学]