Effects of Shenmai and Aminophylline on Apoptosis of Small Airway Smooth Muscle Cells and the Expression of Relevant Genes in Rats with Emphysema  

作　　者：牛汝楫 刘辉国 傅娟 

机构地区：[1]DepartmentofRespiratoryMedicine

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2002年第4期310-312,共3页华中科技大学学报（医学英德文版）

基　　金：This project was supported by a grant from Natural Sci-ences Foundation of China(No.396 70 338)

摘　　要：The effects of Shenmai injection (SMI) and am inophylline on apoptosis of sm all airway smooth muscle cells (SASMC) and the Fas/ Fas L expression in rats with papain- induced em physe- ma were investigated.Rat emphysema model was established by a single intratracheal instillation of papain.Apoptosis and Fas/Fas L expression of SASMC were detected by im munohistochemistry SABC and TU NEL assay at day1,3,5 ,7,15 ,30 after modeling,and the effect of SMI and am inophylline on them were observed.The results indicated that the Fas/Fas L expression positive rate in SASMC was2 .31± 0 .5 5 /1.2 8± 0 .4 7respectively.After a single intratracheal instillation of papain,the expression of Fas/Fas L positive rate in the placebo group was increased in a tim e- dependent manner.SMI could inhibit the expression of Fas/ Fas L ,but aminophylline couldn't. The positive rate of apoptosis in the control group was 0 .87± 0 .32 .After a single intratracheal in- stillation of papain,the SASMC apoptosis positive rate in the placebo group was increased in a tim e- dependent manner.The SASMC apoptosis rate in all groups was declined after treatment with SMI,but the effect of am inophylline was notobvious.Itwas dem onstrated thatin the patho- genesis of emphysem a Fas/Fas L played an important role in the regulation of SASMC apoptosis. SMI influenced the expression of Fas/ Fas L and declined SASMC apoptosis by inhibiting the releas- ing of inflamm atory m edia and played an im portant role in the therapy of em physema.The effects of Shenmai injection (SMI) and am inophylline on apoptosis of sm all airway smooth muscle cells (SASMC) and the Fas/ Fas L expression in rats with papain- induced em physe- ma were investigated.Rat emphysema model was established by a single intratracheal instillation of papain.Apoptosis and Fas/Fas L expression of SASMC were detected by im munohistochemistry SABC and TU NEL assay at day1,3,5 ,7,15 ,30 after modeling,and the effect of SMI and am inophylline on them were observed.The results indicated that the Fas/Fas L expression positive rate in SASMC was2 .31± 0 .5 5 /1.2 8± 0 .4 7respectively.After a single intratracheal instillation of papain,the expression of Fas/Fas L positive rate in the placebo group was increased in a tim e- dependent manner.SMI could inhibit the expression of Fas/ Fas L ,but aminophylline couldn't. The positive rate of apoptosis in the control group was 0 .87± 0 .32 .After a single intratracheal in- stillation of papain,the SASMC apoptosis positive rate in the placebo group was increased in a tim e- dependent manner.The SASMC apoptosis rate in all groups was declined after treatment with SMI,but the effect of am inophylline was notobvious.Itwas dem onstrated thatin the patho- genesis of emphysem a Fas/Fas L played an important role in the regulation of SASMC apoptosis. SMI influenced the expression of Fas/ Fas L and declined SASMC apoptosis by inhibiting the releas- ing of inflamm atory m edia and played an im portant role in the therapy of em physema.

关 键 词：EMPHYSEMA small airway smooth muscle cells FAS Fas L APOPTOSIS 

分 类 号：R563.3[医药卫生—呼吸系统]