增液汤抑制幼鼠胸腺细胞凋亡作用的机制探讨  

Mechanism of Inhibitory Effect of Zengyetang on Thymocyte Apoptosis in Young Rats

作　　者：王君[1] 仝小林[1] 李纯[1] 王坤[1] 迟永春[1] 叶智文[1] 杨梦兰[1] 

机构地区：[1]中日友好医院,北京100029

出　　处：《中国中西医结合杂志》2003年第1期35-39,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助课题 (No .39670 866)

摘　　要：目的 :观察预防性应用增液汤注射剂对幼鼠胸腺细胞凋亡及其相关基因的影响。方法 :给 4～ 5周龄Wistar大鼠腹腔注射增液汤注射剂和地塞米松 ,采用原位末端标记 (TUNEL)法分析不同处理组的凋亡细胞 ,同时采用免疫组化方法检测幼鼠胸腺细胞bcl 2和bax基因蛋白表达情况。结果 :用TUNEL法标记凋亡细胞 ,荧光显微镜下 ,地塞米松组可见致密浓染的黄绿色荧光 ,呈局灶状分布 ;而增液汤组只有散在的荧光。光镜观察地塞米松组TUNEL阳性细胞数目较多 ,其凋亡指数为 0 4 1± 0 0 1;增液汤组TUNEL阳性细胞数目较少 ,凋亡指数为 0 0 7± 0 0 0 4 ,与地塞米松组比较 ,差异有显著性 (P <0 0 1)。免疫组织化学结果表明 ,地塞米松组bcl 2基因蛋白呈低表达 ,其蛋白阳性率为 (0 196 0± 0 0 0 6 0 ) % ,bax蛋白过度表达 ,蛋白阳性率为 (0 4 315± 0 0 16 5 ) % ,bcl 2 /bax <1;相反增液汤组细胞bcl 2基因蛋白呈高表达 ,bax基因蛋白仅有少量表达 ,其蛋白阳性率为 (0 5 0 10± 0 0 170 ) %和 (0 185 4± 0 0 0 9) % ,bcl 2 /bax >1。两组比较 ,差异有显著性 (P <0 0 1)。结论 :通过调控凋亡基因bcl 2 /bax表达 ,增强胸腺细胞对地塞米松的抵抗 ,进而抑制细胞凋亡 ,可能是增液汤抑制幼鼠胸腺细胞凋亡的重要作用环?Objective: To observe the effect of prophylactic application of Zengyetang (ZYT) Injection on thymocyte apoptosis in young rats. Methods: Intraperitoneal injection of ZYT or dexamethasone (DXM) was given to Wistar rats, 4～5 weeks old in different treated groups. Cell apoptosis was determined by TUNEL, and the expressions of bcl 2 and bax genes in thymocytes were detected using immunohistochemical method. Results: After apoptotic cells had been labelled by TUNEL, fluorescent microscopic examination showed that in the DXM group, there was dense deep stained, focal distributed yellow green fluorescence, while in the ZYT group, only scattered fluorescence was shown. Light microscopic observation showed that TUNEL positive cells were higher in the DXM group than those in the ZYT group, the apoptosis index in the former was 0.41±0.01, which was higher than that in the latter (0.07±0.004, P<0 01). Immunohistochemical results showed that in the DXM group, bcl 2 gene protein expression was low with the positive protein rate of 0.1960±0.0060% and bax protein expression was high, with positive protein rate of 0.4315±0.0165%,bcl 2/bax<1. While in the ZYT group, bcl 2 gene protein showed high expression and bax gene protein only showed few expression, the positive protein rate was 0.5010±0 0170% and 0.1854±0.009%, bcl 2/bax>1. The difference between the two groups was significant (P<0 01). Conclusion: One of the important key link of ZYT in inhibiting thymocyte apoptosis in young rats is possibly through regulating apoptotic gene bcl 2/bax expression and enhancing the resistance to DXM, thus to inhibit the apoptosis of thymocytes.

关 键 词：增液汤 肾上腺皮质激素类 细胞凋亡 中药 药理 

分 类 号：R285.5[医药卫生—中药学]