均匀设计优选微波辅助萃取刺五加中有效成分的研究  

Study on microwave-assisted extraction process for active ingredients in Radix acanthopanacis senticosi

作　　者：王娟[1] 沈平孃 沈永嘉[1] 

机构地区：[1]华东理工大学化学与制药学院,上海200237 [2]国家中药制药工程技术研究中心,上海201203

出　　处：《中成药》2003年第1期11-14,共4页Chinese Traditional Patent Medicine

基　　金：国家重点科技攻关项目 (项目编号 99 92 9 0 2 14 )

摘　　要：目的 :以总皂甙和异秦皮啶的含量为指标 ,研究微波辅助萃取刺五加的提取工艺。方法 :通过均匀设计的方法 ,在小试设备上采用连续微波辅射方式进行微波萃取工艺的优化 ;同时在中试设备上也进行了初步研究。结果 :微波功率、微波辐射时间、溶剂浓度、溶剂用量、原料粉碎度、原料浸泡时间等工艺参数对刺五加中有效成分的提取具有交互作用。结论 :小试设备上优选出的最佳工艺为 :微波功率 51 0W、微波辐射时间 30min、溶剂浓度 85 %、固液比例 1∶5、原料粉碎度 40目、浸泡时间 0 .5h ;且通过验证实验表明 ,所建立的模型是合理的。中试研究结果表明 ,该技术应用于中药有效成分的提取是可行的。Objective: To study the microwave assisted extraction process for saponins and isofraxidin in Radix Acanthopanacis Senticosi as a marker. Methods: Using the uniform design, the optimization process of microwave assisted extraction for saponins and isofraxidin in Radix Acanthopanacis Senticosi was gained by continuous microwave radiation. Results: Some parameters such as microwave power, radiation time, solvent concentraction, solvent consumption, pulverized degree and dipping time had interactions on the extraction of active constituents in Radix Acanthopanacis Senticosi. Conclusion: The optimization process was obtained which could be described as following: 510W as the microwave power, 30 min as the radiation time, 85% ethanol as the solvent, 1∶5 as the proportion of raw material to solvent, 40 mesh as the grinding degree and 0.5 hr as the dipping time. Meanwhile, the model developed were proved to be reasonable by the pilot experiment.

关 键 词：总皂苷 异秦皮啶 微波辅助萃取 刺五加 均匀设计 中药 

分 类 号：R284.3[医药卫生—中药学]