TLC-HPLC法分析榧属植物叶中的紫杉醇  

A TLC-HPLC method to analyze taxol in leaf of Torreya Arn.plant

作　　者：张虹[1] 陈振德[1] 柳正良[1] 

机构地区：[1]第二军医大学药学院药物分析学教研室,上海200433

出　　处：《第二军医大学学报》2003年第1期106-107,共2页Academic Journal of Second Military Medical University

摘　　要：榧属植物叶的提取物经 TL C法初步分离 ,再用 HPL C法检测其中紫杉醇的含量。 HPL C测定条件为 Shim- PackCL C- ODS柱 (15 0 m m× 6 .0 mm ,5μm) ;甲醇∶水 (6 6∶ 34)为流动相 ;流速 0 .5 m l/m in,检测波长 2 2 7nm,柱温 35℃。该法在10 0～ 5 0 0μg/ml浓度范围内峰高 (Y)与其浓度 (X)具有良好的线性关系 ;平均回收率为 85 .7% (n=12 ) ,RSD为 7.8% ;精密度、重复性较好。A TLC condition has been developed to purify the extraction from the leaf of Torreya Arn. plant,then an optimized HPLC method was used to determine the amount of taxol.HPLC chromatographic conditions were:colum:Shim Pack CLC ODS(150 mm×6.0 mm,5 μm);mobile phase:methanol water(66∶34);flow rate: 0.5 ml/min;wavelength of the detector: 227 nm;colum temperature: 35℃.Using this method,we have determined the trace level of taxol in the leaf of Torreya Arn .plant successfully.There exists better linear relationship between peak height( Y ) and the concentration of detected sample( X ) within 100 500 μg/ml.The average recovery rate is 85.7%( n =12);RSD is 7.8%.

关 键 词：薄层色谱法 高效液相色谱法 紫杉醇 榧属植物中药 

分 类 号：R282.71[医药卫生—中药学]