冠心病患者载脂蛋白E、血管紧张素转换酶联合基因型研究  

EFFECT OF APOLIPOPROTEIN E,ANGIOTENSIN CONVERTING ENZYME GENE ON CORONARY HEART DISEASE IN CHINESE POPULATION

作　　者：李红梅[1] 潘闽[1] 梁舒[1] 王惠民[2] 崔之础[1] 朱健华[1] 

机构地区：[1]南通医学院附属医院心内科,南通226001 [2]南通医学院附属医院酶学研究室

出　　处：《中国现代医学杂志》2003年第2期35-37,共3页China Journal of Modern Medicine

摘　　要：目的 :研究中国人载脂蛋白E(ApoE)基因多态性及血管紧张素转换酶 (ACE)基因插入 /缺失 (I/D)多态性与冠心病的关联。方法 :分别用PCR -RFLP及PCR技术检测了 12 9例冠心病患者及 90例健康人ApoE ,ACE基因型。结果 :冠心病组ε3 / 4基因型及ε4等位基因频率显著高于对照组 ,ε3 / 3 基因型及ε3 等位基因频率则显著低于对照组 ;ACE各基因型及等位基因频率两组间无显著差异 ;在ε4携带情况下 ,不同ACE基因型冠心病危险度不同 ,DD型 >ID型 ,而Ⅱ型似有一定的保护作用。结论 :ε4等位基因是冠心病的遗传易患因子 ;ACE基因与冠心病发病无显著关联 ,但对ε4等位基因有一定的“修饰”作用。Objective: To determine the relevance of apolipoprotein E(ApoE),angiotensin converting enzyme(ACE) gene polymorphism for coronary heart disease(CHD).Methods:Genotypes of ApoE and ACE of 129 cases of CHD and 90 case normal people were detected by PCR-RFLP and PCR respectively. Results:The frequencies of ε 3/4 genotypes and ε 4 alleles in CHD were significantly higher than those of control group, frequencies of ε 3/3 and ε 3 were lower, there were no differences of ACE genotypes and alleles frequencies between two groupes, but various ACE genotypes had different CHD risk when carrying ε 4 allele. Conclusion: ε 4 allele is a genetic marker of CHD.ACE gene has no relationship to CHD, but has modifying effect on ε 4 allele.

关 键 词：载脂蛋白E 血管紧张素转换酶 基因 冠心病 PCR技术 

分 类 号：R541.4[医药卫生—心血管疾病]