脑宁康颗粒对脑缺血预适应大鼠CD 62P水平的影响  

Effect of Naoningkang particle(脑宁康颗粒) on level of CD 62P of rats treated ischemic preconditioning

作　　者：魏江磊[1] 

机构地区：[1]上海曙光医院,上海200021

出　　处：《中国中西医结合急救杂志》2003年第1期29-30,共2页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：山东省科委科研基金资助项目 (J98K5 5 )

摘　　要：目的 :观察中药脑宁康 (NNK)颗粒对经脑缺血预适应 (IPC)处理后的大鼠血小板膜表面受体(CD6 2 P)水平的影响。方法 :根据 Pulsinelli四管法及 L iu法分别制作大鼠 IPC及脑缺血再灌注 (IR)模型 ;以荧光抗体标记法在流式细胞仪上测定并比较 NNK颗粒对 IPC和 IR两种模型大鼠 CD6 2 P水平的影响。结果 :IPC模型各组 CD6 2 P均较 IR模型变化幅度小 ,其中 IPC方法与 IR方法模型组间有显著差异 (P<0 .0 5 )。在IPC和 IR两种模型中 ,NNK组及对照组药物阿斯匹林 (ASP)均对 CD6 2 P有良性效应 ,其中 NNK组分别为(2 .0 6± 0 .0 6 ) % (IPC法 )及 (1.98± 0 .0 5 ) % (IP法 ) ,略优于 ASP的 (2 .16± 0 .0 7) % (IPC法 )及 (2 .0 3±0 .0 5 ) % (IP法 ) ,但均无显著性差异 (P均 >0 .0 5 )。结论 :NNK颗粒对两种模型均有稳定调整 CD 6 2 P的效应 ,其强度与 ASP持平 ,其机制可能与诱导和强化 IPC作用、从而保护脑神经元有关。Objective:To observe the effect of Naoningkang(NNK) particles(脑宁康颗粒) on the receptor of CD62P blood platelet surface membrane after rats treated ischemic preconditioning (IPC).Methods:The rats' IPC and ischemic reperfusion(IR) models were made by methods of Pulsinelli four tubes and Liu respectively. Level of receptor of platelet membrane CD 62P was observed before and after administrated NNK by flow cytometer and method of fluorescent antibody label.Results:The changes of CD 62P in IPC model groups were smaller than those in IR model groups.There was significantly different in IPC pathological model group( P <0 05).There had efficacy on CD 62P in NNK and asprine groups.It showed (2 06±0 06)% (IPC model) and (1 98±0 05)%(IP model) in two NNK groups,better than those(2 16±0 07)%( IPC model ) and (2 03±0 05)%(IP model) in two ASP groups,while there was no significantly difference. Conclusion: NNK can adjust CD 62P level stability on two kinds of models and its effect is similar to ASP.So NNK may have function to induce and tonify IPC and protect brain neuron.

关 键 词：脑缺血 脑宁康颗粒 缺血预适应 血小板膜表面受体CD62P 动物模型 

分 类 号：R285.5[医药卫生—中药学]