植物生物分子的电分析化学研究——Ⅸ.多酚氧化酶活性的脉冲极谱法测定  

Electroanalytical Studies of Plant Biological Molecules Ⅸ. The Differential Pulse Polarographic Determination of Polyphenols Oxidase Activity

作　　者：汪乃兴 赵滨[1] 

机构地区：[1]复旦大学化学系,上海200433

出　　处：《分析化学》1992年第12期1442-1444,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：本文用微分脉冲极谱法研究了多酚氧化酶的活性测定和酶的反应动力学。应用于马铃薯等10余种植物样品的酶活力测定,结果良好。方法简便快速,重现性好。The determination of polyphenols oxidase activity and the effects of various factors on the activity have been investigated by differential pulse polarography. The results show a good agreement with those obtained by titrimetric method. This method provides a rapid and simple procedure for direct determination of polyphenols oxidase activity and is also successfully applied in several kinds of plant samples such as potato, mushroom, caulif lower,water chestnut,radish,apple,banana,etc..

关 键 词：多酚氧化酶 脉冲极谱法 

分 类 号：Q946.54[生物学—植物学]