海马NMDA受体在大鼠严重烫伤后HPA轴兴奋性变化中的作用  

Effects of hippocampal NMDA receptor on HPA axis activity following severe burn

作　　者：陆建华[1] 宋艳玲[2] 黎海蒂[1] 高京生[3] 赵邦云[1] 

机构地区：[1]第三军医大学基础医学部生理学教研室,重庆400038 [2]兰州军区乌鲁木齐总医院干部病房,乌鲁木齐830000 [3]第三军医大学预防医学系防原医学教研室,全军复合伤研究所,重庆400038

出　　处：《第三军医大学学报》2003年第3期216-218,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目 ("973项目 ) (G19990 54 2 0 1)

摘　　要：目的　研究海马N 甲基 D 天冬氨酸受体 (NMDA受体 ,NR)在创伤导致的HPA轴过度兴奋中的作用。方法　以大鼠背部 3 0 %TBSAⅢ度烫伤作为严重创伤过度应激模型 ,分别观察海马内微量注射NR的拮抗剂MK 80 1或NR的功能受体NR1的反义寡核酸 (Oligodeoxynucleotide ,ODN)对烫伤后 2h血清ACTH及皮质醇浓度的影响。结果　严重烫伤后2h ,血清ACTH及皮质醇浓度明显升高 ;与烫伤组相比 ,海马内微量注射生理盐水 ,没有明显改变烫伤后血清ACTH及皮质醇浓度 ;海马内微量注射MK 80 16μg可明显降低烫伤后血清ACTH及皮质醇浓度 ;注射MK 80 112 μg可进一步降低血清ACTH及皮质醇浓度 ;注射NR1反义ODN 10nmol L可明显降低烫伤后血ACTH及皮质醇浓度 ;注射 2 0nmol L可进一步降低血清ACTH及皮质醇浓度。结论　海马NR参与了烫伤HPA轴的过度激活。Objective To investigate the changes of HPA axis activity following scald stress and to elucidate if NMDA receptor is involved in this change. Methods Adult male Wistar rats were inflicted with 30% TBSA full thickness scalding burn, which was applied as severe trauma stress model. Using this model, we detected the changes of serum cortisol and ACTH concentration in scald rats pretreated with intrahippocampal microinjection of NMDA receptor antagonist MK801 or NMDA receptor 1 antisense oligodeoxynucleotide. Results Intrahippocampal microinjection of MK 801 6 μg resulted in an significant decrease of serum cortisol and ACTH concentration at 2 h after burn, and microinjection of MK 801 12 μg resulted in a more significant decrease of these values. In accordance with microinjection of MK 801, intrahippocampal microinjection of NMDA receptor 1 antisense oligodeoxynucleotide of 10 nmol/L and 20 nmol/L also resulted in a significant and a more significant decrease of serum cortisol and ACTH concentration after burn. Conclusion Hippocampal NMDA receptor plays an important role in over excitation of HPA axis following burn.

关 键 词：烧伤 应激 海马 NMDA受体 HPA轴 

分 类 号：R644[医药卫生—外科学]