低水平血清乙型肝炎病毒表面抗原、e抗原测定及其临床意义  

Clinical evaluation of low level HBsAg and HBeAg in serum

作　　者：李君[1] 臧桂珍[1] 李鹤林[1] 杨松梅[1] 

机构地区：[1]东南大学医学院附属南京第二医院检验科,江苏南京210003

出　　处：《东南大学学报（医学版）》2003年第1期41-43,共3页Journal of Southeast University(Medical Science Edition)

摘　　要：目的 :了解低水平乙型肝炎 (乙肝 )病毒表面抗原 (HBsAg)、e抗原 (HBeAg)在乙肝患者中的分布和相关乙肝病毒血清标志物的模式特征。方法 :应用微粒子酶免疫技术 (MEIA)对 790 0例门诊和住院病人血清标本进行乙肝病毒 5项标志物的检测 ,根据临界值质控血清的值来确定质量浓度在 5 μg·L-1或 1μg·L-1以下HBsAg阳性数 ,以及浓度在 2nCu·ml-1以下HBeAg阳性数 ,并分析相关乙肝病毒标志物 (HBV M )的模式。结果 :共检出HBsAg阳性 6162例 ,其中质量浓度在 5 μg·L-1以下者占13 .5 3 % ,1μg·L-1以下者 8.4% ;HBeAg阳性者 44 0 1例 ,其中浓度在 2nCu·ml-1以下者为 2 1.9%。 5项血清标志物检测发现不少特殊模式 ,HBsAg与抗 HBs同时阳性者主要出现在HBsAg质量浓度低于 1μg·L-1的标本中 ;血清HBeAg浓度在 2nCu·ml-1以下者 ,HBeAg和抗 HBe同时阳性的占 5 2 .4%。结论 :提高HBsAg、HBeAg检测的灵敏度有助于乙肝的诊断、治疗以及流行病学调查 。Objective To investigate the distribution of the population with low level HBsAg,HBeAg in serum and the characteristics of their HBV serologic markers.Methods AxSyM microparticle enzyme immunoassay(MEIA) system was used to detect contents of the five markers in the serum samples from 7?900 patients and determine the patients with HBsAg concentration less than 5?μg·L -1 ,1?μg·L -1 .Results There were 6?162 patients with positive HBsAg,including 13.53%, 8.4 % with HBsAg less than 5?μg·L -1 ,less than 1?μg·L -1 respectively.And there were 4?401 patients with positive HBeAg,including 21.9% with HBeAg less than 2?nCu·ml -1 .There also had many special types.HBsAg and HBs antibodies positive were frequently present in those with HBsAg concentration less than 1?μg·L -1 .HBeAg and HBe antibodies were positive in 52.4 % patients with HBeAg concentration less than 2?nCu·ml -1 .Conclusions Enhancing the detecting sensitivity of HBsAg and HBeAg is helpful in diagnosis,treatment and epidemiologic investigation of hepatic patients.A define answer can be attained by MEIA in patients with nu causing high aminotransferase.

关 键 词：血清 乙型肝炎病毒 表面抗原 E抗原 测定 

分 类 号：R512.62[医药卫生—内科学]