聚合酶链反应和DNA探针杂交法检测沙眼衣原体的比较  

Polymerase chain reaction vs DNA probe hybridization for detecting Chlamydia trachomatis in female urogenital tract

作　　者：朱军[1] 王卫萍[2] 陈亚利[2] 武建国[2] 

机构地区：[1]东南大学附属中大医院检验科,江苏南京210009 [2]南京军区南京总医院全军医学检验中心,江苏南京210002

出　　处：《东南大学学报（医学版）》2003年第1期35-37,共3页Journal of Southeast University(Medical Science Edition)

摘　　要：目的 :探讨聚合酶链反应 (PCR)和DNA探针杂交法检测泌尿生殖道沙眼衣原体 (Ct)的临床诊断价值。方法 :应用这两种方法对未使用过抗衣原体药物的 80例宫颈炎患者同时进行Ct检测。结果 :PCR法检出Ct基因的阳性率 (2 3 .8% )高于DNA探针杂交法 (15 .0 % ) ,两者比较有显著性差异。结论 :PCR法检测Ct较DNA探针杂交法敏感 。Objective To explore the clinical diagonstic value of polymerase chain reaction (PCR) and DNA probe hybridization for detecting Chlamydia trachomatis (Ct) infection of urogenital tract.Method 80 specimens from cervicitis subject were examined by PCR and DNA probe hybridization simultaneously.Result The positive rate of PCR (23.8%) was higher than that of DNA probe hybridization (15.0%) significantly.Conclusion PCR is more sensitive than DNA probe hybridization in detecting Ct infection,and the combination of two would be more preferable.

关 键 词：沙眼衣原体 宫颈炎 聚合酶链反应 DNA探针 核酸杂交 

分 类 号：R711.32[医药卫生—妇产科学]